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Literature summary extracted from

  • Shaw, P.D.
    Biosynthesis of nitro-compounds. III. The enzymatic reduction of beta-nitroacrylic acid to beta-nitropropionic acid (1967), Biochemistry, 6, 2253-2260.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.16 additional information enzyme is inhibited by sulfhydryl compounds, e.g. cysteine at concentrations equivalent to 3-nitroacrylic acid, inhibition probably due to the reaction of the sulfhydryl compounds with 3-nitroacrylic acid rather than an inhibition of the enzyme itself Penicillium atrovenetum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.16 0.037
-
 NADPH
-
 Penicillium atrovenetum
1.3.1.16 0.16
-
 3-Nitroacrylate
-
 Penicillium atrovenetum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.16 3-nitroacrylate + NADPH Penicillium atrovenetum biosynthesis of beta-nitropropionic acid 3-nitropropanoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.16 Penicillium atrovenetum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.16 partial Penicillium atrovenetum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.16 mycelium
-
 Penicillium atrovenetum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.16 0.866
-
-
 Penicillium atrovenetum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.16 3-nitroacrylate + NADPH highly specific Penicillium atrovenetum 3-nitropropanoate + NADP+
-
 ?
1.3.1.16 3-nitroacrylate + NADPH biosynthesis of beta-nitropropionic acid Penicillium atrovenetum 3-nitropropanoate + NADP+
-
 ?
1.3.1.16 additional information no substrates are acrylic acid, crotonic acid, fumaric acid, cinnamic acid, 2-hydroxy-3-nitropropionic acid, shikimic acid, aconitic acid, itaconic acid, pyruvic acid, phosphoenolpyruvate, cytochrome c Penicillium atrovenetum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.16 5
-
-
 Penicillium atrovenetum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.16 3 7 at pH 3 about 35% of activity maximum, at pH 7 about 60% of activity maximum Penicillium atrovenetum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.16 NADP+
-
 Penicillium atrovenetum
1.3.1.16 NADPH NADH cannot replace NADPH Penicillium atrovenetum