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Literature summary extracted from

  • Friedmann, H.C.; Krakow, G.
    Bestimmung mit Dihydroorotat-Dehydrogenase (1984), Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. ), 2, 2010-2014.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.14 cysteine required for activation Faecalicatena orotica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.14 5-Methylorotate 2 mM, 50% inhibition of reduction of orotate Faecalicatena orotica
1.3.1.14 isopropyl hydantoin
-
 Faecalicatena orotica
1.3.1.14 Na3PO4 0.2 M, at pH 6.5, 20% inhibition Faecalicatena orotica
1.3.1.14 NaCl 0.2 M, 55% inhibition Faecalicatena orotica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.14 0.13
-
 Orotate
-
 Faecalicatena orotica
1.3.1.14 0.13
-
 5-Fluoroorotate
-
 Faecalicatena orotica

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.14 Faecalicatena orotica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.14
-
 Faecalicatena orotica

Storage Stability

EC Number Storage Stability Organism
1.3.1.14 above 20°C, 0.2 M phosphate buffer, fast loss of activity Faecalicatena orotica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.14 (S)-dihydroorotate + NAD+
-
 Faecalicatena orotica orotate + NADH + H+
-
 r
1.3.1.14 5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
 r
1.3.1.14 additional information not: uracil, cytosine, 5-methylcytosine, thymine as substrates Faecalicatena orotica ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.14 additional information
-
 additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.14 6.5
-
 maximal activity Faecalicatena orotica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.14 FAD flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
1.3.1.14 flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
1.3.1.14 FMN flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
1.3.1.14 additional information contains 2 mol of iron per mol of enzyme Faecalicatena orotica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.14 3.3
-
 isopropyl hydantoin
-
 Faecalicatena orotica