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Literature summary extracted from

  • Ikebukuro, K.; Wakamura, H.; Karube, I.
    Phosphate sensing system using pyruvate oxidase and chemiluminescence detection (1996), Biosens. Bioelectron., 11, 959-965.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.2.3.3 analysis rapid detection of phosphate using immobilized pyruvate oxidase Pediococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.3 pyruvate + phosphate + O2 + H2O Pediococcus sp.
-
 acetyl phosphate + CO2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.3 Pediococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.3.3 ion exchange column chromatography, immobilization on aminoalkylated controlled pore glass by crosslinking with glutaraldehyde Pediococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.3 pyruvate + phosphate + O2 + H2O
-
 Pediococcus sp. acetyl phosphate + CO2 + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.3.3 FAD
-
 Pediococcus sp.
1.2.3.3 thiamine diphosphate
-
 Pediococcus sp.