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Literature summary extracted from

  • Bittner, F.; Oreb, M.; Mendel, R.R.
    ABA3 is a molybdenum cofactor sulfurase required for activation of aldehyde oxidase and xanthine dehydrogenase in Arabidopsis thaliana (2001), J. Biol. Chem., 276, 40381-40384.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.1 expression in Pichia pastoris of the AOalpha gene Arabidopsis thaliana
2.8.1.9 in vector pQE80 Arabidopsis thaliana

General Stability

EC Number General Stability Organism
1.2.3.1 recombinant enzyme inactivated by KCN treatment Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.9 N-ethylmaleimide 4fold excess, 71% inhibition Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.9 92600
-
 calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.3.1 abscisic aldehyde + H2O + O2 Arabidopsis thaliana four aldehyde oxidases are known that have varying affinities to abscisic aldehyde. AOdelta, endcoded by AAO3 specifically catalyses this step in rosette leaves abscisic acid + H2O2
-
 ?
2.8.1.9 MoO2(OH)Dtpp-mP + L-cysteine + 2 H+ Arabidopsis thaliana
-
 MoOS(OH)Dtpp-mP + L-alanine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Arabidopsis thaliana
-
-
-
2.8.1.9 Arabidopsis thaliana Q9C5X8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.9 nickel-nitrilotriacetic acid superflow matrix, UNO Q-1 column, Superose 12 size exclusion column Arabidopsis thaliana

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.3.1 after inactivation with KCN, anaerobic reconstitution by sulfide and dithionite were succesful Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.9 leaf highest levels in leaves and roots Arabidopsis thaliana
-
2.8.1.9 root highest levels in leaves and roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 abscisic aldehyde + H2O + O2 four aldehyde oxidases are known that have varying affinities to abscisic aldehyde. AOdelta, endcoded by AAO3 specifically catalyses this step in rosette leaves Arabidopsis thaliana abscisic acid + H2O2
-
 ?
1.2.3.1 indole 3-carbaldehyde + H2O + O2
-
 Arabidopsis thaliana indole 3-carboxylate + H2O2
-
 ?
2.8.1.9 MoO2(OH)Dtpp-mP + L-cysteine + 2 H+
-
 Arabidopsis thaliana MoOS(OH)Dtpp-mP + L-alanine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 AO
-
 Arabidopsis thaliana
2.8.1.9 ABA3
-
 Arabidopsis thaliana
2.8.1.9 molybdenum cofactor sulfurase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.9 pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.8.1.9 metabolism molybdenum cofactor biosynthesis Arabidopsis thaliana