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Literature summary extracted from
- Betaine aldehyde dehydrogenase in spinach (1988), Bot. Bull. Acad. Sin., 29, 255-263.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.8 | NaCl | inhibitory effect increases with concentrations from 50 mM to 250 mM | Spinacia oleracea |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.8 | 40000 | - |
1 * 40000 + 1 * 64000, BADH II, SDS-PAGE | Spinacia oleracea |
| 1.2.1.8 | 64000 | - |
1 * 40000 + 1 * 64000, BADH II, SDS-PAGE | Spinacia oleracea |
| 1.2.1.8 | 98000 | - |
gel filtration | Spinacia oleracea |
| 1.2.1.8 | 104000 | - |
BADH I and BADH II, disc gradient gel electrophoresis | Spinacia oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.8 | Spinacia oleracea | - |
2 enzyme forms: BADG I and BADH II. BADH II comprises more than 60% of the total activity | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.8 | 2 enzyme forms: BADH I and BADH II | Spinacia oleracea |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.8 | 0.075 | - |
BADH I | Spinacia oleracea |
| 1.2.1.8 | 0.311 | - |
BADH II | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.8 | betaine aldehyde + NAD+ + H2O | - |
Spinacia oleracea | betaine + NADH | - |
? | |
| 1.2.1.8 | betaine aldehyde + NADP+ + H2O | no activity with NAD+ | Spinacia oleracea | betaine + NADPH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.8 | dimer | 1 * 40000 + 1 * 64000, BADH II, SDS-PAGE | Spinacia oleracea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.8 | 45 | - |
- |
Spinacia oleracea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.8 | 8.5 | 9 | BADH I and BADH II | Spinacia oleracea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.8 | NAD+ | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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