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Literature summary extracted from

  • Abadjieva, A.; Pauwels, K.; Hilven, P.; Crabeel, M.
    A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis. Acetylglutamate synthase activity requires complex formation with acetylglutamate kinase (2001), J. Biol. Chem., 276, 42869-42880.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.1 additional information enzyme must interact stoichoimetrically in vivo with acetylglutamate kinase to be active Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.1 coexpression of N-acetylglutamate synthase gene ARG2 together with ARG5 and ARG6 coding for acetylglutamate kinase and acetylglutamyl-phosphate reductase in Saccharomyces cerevisiae Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 52000
-
 x * 52000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.1 acetyl-CoA + L-glutamate Saccharomyces cerevisiae enzyme catalyzes the first step in arginine biosynthesis CoA + N-acetyl-L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.38 Saccharomyces cerevisiae
-
-
-
2.3.1.1 Saccharomyces cerevisiae
-
-
-
2.7.2.8 Saccharomyces cerevisiae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.8 ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate enzyme has to interact stoichiometrically with acetylglutamate synthase in order to be active Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.38 N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate
-
 Saccharomyces cerevisiae N-acetyl-5-glutamyl phosphate + NADPH
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate
-
 ?
2.3.1.1 acetyl-CoA + L-glutamate enzyme catalyzes the first step in arginine biosynthesis Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Saccharomyces cerevisiae ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.8 ? x * 52000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.38 NADP+
-
 Saccharomyces cerevisiae