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Literature summary extracted from

  • Quayle, J.R.
    Glyoxylate dehydrogenase (1966), Methods Enzymol., 9, 342-346.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.17 Tris buffer
-
 Cupriavidus oxalaticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.17 glyoxylate + CoA + NADP+ Pseudomonas sp.
-
 oxalyl-CoA + NADPH
-
 r
1.2.1.17 glyoxylate + CoA + NADP+ Cupriavidus oxalaticus
-
 oxalyl-CoA + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.17 Cupriavidus oxalaticus
-
-
-
1.2.1.17 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.17 using protamine sulfate treatment, ammonium sulfate precipitation and dialysis, alumina C-gamma adsorption, concentration of the enzyme by ammonium sulfate precipitation followed by dialysis and ion exchange chromatography on DEAE-cellulose Cupriavidus oxalaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.17 4.2
-
-
 Cupriavidus oxalaticus
1.2.1.17 7.2
-
-
 Cupriavidus oxalaticus

Storage Stability

EC Number Storage Stability Organism
1.2.1.17 -15°C, several weeks Cupriavidus oxalaticus
1.2.1.17 0°C, 70% loss of activity, 24 h Cupriavidus oxalaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.17 glyoxylate + CoA + NADP+
-
 Pseudomonas sp. oxalyl-CoA + NADPH
-
 r
1.2.1.17 glyoxylate + CoA + NADP+
-
 Cupriavidus oxalaticus oxalyl-CoA + NADPH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.17 oxalyl-CoA reductase
-
-

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.17 8.6
-
 glyoxylate + CoA + NADP+ Cupriavidus oxalaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.17 CoA
-
 Pseudomonas sp.
1.2.1.17 CoA may be replaced by pantetheine, N-acetylcysteamine reacts very slowly in place of CoA Cupriavidus oxalaticus
1.2.1.17 NADP+
-
 Pseudomonas sp.
1.2.1.17 NADP+ specific for Cupriavidus oxalaticus