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Literature summary extracted from
- Purification and characterization of aspartic -semialdehyde dehydrogenase from yeast and purification of an isozyme of glyceraldehyde-3-phosphate dehydrogenase (1973), Biochemistry, 12, 2264-2270.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.11 | 0.036 | - |
NADP+ | - |
Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.11 | 41000 | - |
4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
| 1.2.1.11 | 156000 | - |
sedimentation equilibrium, meniscus depletion technique | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.11 | L-4-aspartyl phosphate + NADPH | Saccharomyces cerevisiae | synthesis of threonine | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.11 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.11 | using protamine sulfate treatment, ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-50 column, Bio-Gel hydroxylapatite phosphate column, Bio-Gel hydroxylapatite sulfate column, acid treatment and column chromatography on Sephadex G-200 | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.11 | 73 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.11 | L-4-aspartyl phosphate + NADPH | synthesis of threonine | Saccharomyces cerevisiae | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
? | |
| 1.2.1.11 | L-aspartate 4-semialdehyde + phosphate + NADP+ | - |
Saccharomyces cerevisiae | L-4-aspartyl phosphate + NADPH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.11 | tetramer | 4 * 41000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.11 | NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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