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Literature summary extracted from

  • Christmas, P.; Ursino, S.R.; Fox, J.W.; Soberman, R.J.
    Expression of the CYP4F3 gene. Tissue-specific splicing and alternative promoters generate high and low Km forms of leukotriene B4.omega-hydroxylase (1999), J. Biol. Chem., 274, 21191-21199.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.94 chromosomal localization 19p Homo sapiens
1.14.14.94 CYP4F3 gene contains 14 exons and 13 introns Homo sapiens
1.14.14.94 expression in transfected COS-7 cells Homo sapiens
1.14.14.94 tissue-specific expression is regulated by alternative promoter usage and mutually exclusive exon splicing, exon 3 and 4 Homo sapiens
1.14.14.94 two isoforms of CYP4F3, CYP4F3A expressed in neutrophils and CYP4F3B expressed in fetal and adult liver Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.94 0.004
-
 leukotriene B4 recombinant isoform CYP4F3A, expressed in COS-7 cells Homo sapiens
1.14.14.94 0.105
-
 leukotriene B4 recombinant isoform CYP4F3B, expressed in COS-7 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.94 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.94 61000
-
 recombinant isoforms CYP4F3A and CYP4F3B expressed in COS-7 cells, western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.94 (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens inactivation of leukotriene B4, a mediator of inflammation (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.94 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.94 liver isoform CYP4F3B Homo sapiens
-
1.14.14.94 neutrophil isoform CYP4F3A Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.94 (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2 inactivation of leukotriene B4, a mediator of inflammation Homo sapiens (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.94 leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2 leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate Homo sapiens 20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.94 CYP4F3
-
 Homo sapiens
1.14.14.94 cytochrome P450 4F3
-
 Homo sapiens