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Literature summary extracted from

  • Shinagawa, E.; Ameyama, M.
    D-Sorbitol dehydrogenase from Gluconobacter suboxydans, membrane-bound (1982), Methods Enzymol., 89, 141-145.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.21 30
-
 D-sorbitol
-
 Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.21 cytoplasmic membrane outer surface Gluconobacter oxydans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.21 17000
-
 1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE Gluconobacter oxydans
1.1.99.21 51000
-
 1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE Gluconobacter oxydans
1.1.99.21 63000
-
 1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE Gluconobacter oxydans
1.1.99.21 131000
-
 calculated sum of each MW of the 3 subunits Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.21 D-sorbitol + acceptor Gluconobacter oxydans high specificity L-sorbose + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.21 Gluconobacter oxydans
-
 var. alpha IFO 3254
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.21
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.21 433
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.21 D-mannitol + acceptor oxidation at 5% the rate of D-sorbitol Gluconobacter oxydans ? + reduced acceptor
-
 ?
1.1.99.21 D-sorbitol + acceptor
-
 Gluconobacter oxydans L-sorbose + reduced acceptor
-
 ?
1.1.99.21 D-sorbitol + acceptor high specificity Gluconobacter oxydans L-sorbose + reduced acceptor
-
 ?
1.1.99.21 additional information no oxidation of D-arabitol, L-iditol, meso-erythritol, galactitol, dulcitol, ribitol, xylitol Gluconobacter oxydans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.21 More 1 * 63000 + 1 * 51000 + 1 * 17000, dissociation into 3 components, a flavoprotein (MW 63000), a cytochrome c (MW 51000) and an unknown polypeptide (MW 17000), SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.21 25
-
-
 Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.21 4.5
-
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.21 cytochrome c tightly bound , dehydrogenase-cytochrome c complex, separable by SDS-PAGE Gluconobacter oxydans
1.1.99.21 cytochrome c rapid reduction in the presence of coenzyme Q1 Gluconobacter oxydans
1.1.99.21 FAD
-
 Gluconobacter oxydans
1.1.99.21 additional information NAD+, NADP+ or molecular oxygen are completely inactive Gluconobacter oxydans