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Literature summary extracted from
- Hexopyranoside: cytochrome c oxidoreductase from Agrobacterium (1975), Methods Enzymol., 41, 153-158.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.13 | Ca2+ | not | Agrobacterium tumefaciens |  |
| 1.1.99.13 | cyanide | 70% inhibition at high concentration | Agrobacterium tumefaciens | |
| 1.1.99.13 | additional information | not: N3-, CO, EDTA | Agrobacterium tumefaciens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.13 | 0.2 | - |
cellobiose | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 0.21 | - |
Lactobionate | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 0.36 | - |
Maltobionate | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 1.7 | - |
lactose | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 2.8 | - |
D-maltose | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 2.9 | - |
D-glucose | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 4.1 | - |
sucrose | - |
Agrobacterium tumefaciens | |
| 1.1.99.13 | 25 | - |
D-galactose | - |
Agrobacterium tumefaciens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.13 | cytoplasm | - |
Agrobacterium tumefaciens | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 73000 | - |
gel filtration | Agrobacterium tumefaciens |
| 1.1.99.13 | 85000 | - |
low speed sedimentation | Agrobacterium tumefaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.13 | Agrobacterium tumefaciens | - |
strain ATCC 143 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.13 | - |
Agrobacterium tumefaciens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.99.13 | -18°C, stable for 2 weeks under nitrogen | Agrobacterium tumefaciens |
| 1.1.99.13 | frozen, half-life 1 week | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.13 | 2-deoxy-D-galactose + acceptor | - |
Agrobacterium tumefaciens | 2-deoxy-3-dehydro-D-galactose + reduced acceptor | - |
? | |
| 1.1.99.13 | 2-deoxy-D-glucose + acceptor | - |
Agrobacterium tumefaciens | 2-deoxy-3-dehydro-D-glucose + reduced acceptor | - |
? | |
| 1.1.99.13 | alpha-methyl-D-glucoside + acceptor | - |
Agrobacterium tumefaciens | 3-keto-alpha-methyl-glucoside + reduced acceptor | - |
? | |
| 1.1.99.13 | anhydro-1,6-D-glucose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | beta-melibiose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | cellobionate + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | cellobiose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | D-galactose + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-D-galactose + reduced acceptor | - |
? | |
| 1.1.99.13 | D-glucosamine + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-D-glucosamine + reduced acceptor | - |
? | |
| 1.1.99.13 | D-glucose + acceptor | - |
Agrobacterium tumefaciens | 3-ketoglucose + reduced acceptor | - |
? | |
| 1.1.99.13 | D-glucose 1-phosphate + acceptor | - |
Agrobacterium tumefaciens | 3-keto-D-glucose 1-phosphate + reduced acceptor | - |
? | |
| 1.1.99.13 | D-mannose + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-D-mannose + reduced acceptor | - |
? | |
| 1.1.99.13 | lactobionate + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | lactose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | lactulose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | leucrose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | maltobionate + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | maltose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | melezitose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | methyl-beta-D-glucose + acceptor | - |
Agrobacterium tumefaciens | 3-keto-methyl-beta-D-glucose + reduced acceptor | - |
? | |
| 1.1.99.13 | methyl-beta-thiogalactose + acceptor | - |
Agrobacterium tumefaciens | 3-dehydro-methyl-beta-D-thiogalactose + reduced acceptor | - |
? | |
| 1.1.99.13 | p-arbutine + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | raffinose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.99.13 | sucrose + acceptor | 2,6-dichlorophenolindophenol as electron acceptor | Agrobacterium tumefaciens | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor | - |
? | |
| 1.1.99.13 | sucrose + acceptor | ferricyanide as electron acceptor | Agrobacterium tumefaciens | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor | - |
? | |
| 1.1.99.13 | sucrose + acceptor | cytochrome c from heart muscle as electron acceptor | Agrobacterium tumefaciens | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor | - |
? | |
| 1.1.99.13 | sucrose + acceptor | electron acceptor phenazine methosulfate | Agrobacterium tumefaciens | 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor | - |
? | |
| 1.1.99.13 | trehalose + acceptor | - |
Agrobacterium tumefaciens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.13 | hexopyranoside-cytochrome c oxidoreductase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 30 | - |
enzyme assay | Agrobacterium tumefaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.13 | 6 | - |
lactose with 2,6-dichlorphenolindophenol as acceptor | Agrobacterium tumefaciens |
| 1.1.99.13 | 7 | - |
lactose with cytochrome c as acceptor | Agrobacterium tumefaciens |
| 1.1.99.13 | 8 | - |
lactose with phenazine methosulfate as acceptor | Agrobacterium tumefaciens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.13 | FAD | - |
Agrobacterium tumefaciens | |
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Release 2023.1 (January 2023)
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