Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Neal, R.A.
    Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2-methyl-4-amino-5-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism (1970), J. Biol. Chem., 245, 2599-2604.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.23 Cu2+
-
 unidentified soil bacterium
1.1.3.23 Hg2+
-
 unidentified soil bacterium
1.1.3.23 p-Chloromeruribenzoate
-
 unidentified soil bacterium
1.1.3.23 Quinacrine
-
 unidentified soil bacterium
1.1.3.23 Semicarbazide
-
 unidentified soil bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.23 1.72
-
 thiamine
-
 unidentified soil bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.23 41500
-
 gel filtration unidentified soil bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.23 thiamine + O2 unidentified soil bacterium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.23 unidentified soil bacterium
-
 ATCC 25589
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.23
-
 unidentified soil bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.23 additional information
-
-
 unidentified soil bacterium
1.1.3.23 2.18
-
-
 unidentified soil bacterium

Storage Stability

EC Number Storage Stability Organism
1.1.3.23 -70°C, 3 months unidentified soil bacterium
1.1.3.23 4°C, 1 week unidentified soil bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.23 oxythiamine + O2
-
 unidentified soil bacterium ? + H2O2
-
 ?
1.1.3.23 pyrithiamine + O2
-
 unidentified soil bacterium ? + H2O2
-
 ?
1.1.3.23 thiamine + O2
-
 unidentified soil bacterium thiamine acetate + H2O2
-
 ?
1.1.3.23 thiamine + O2
-
 unidentified soil bacterium ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.23 50
-
 stable for 30 min unidentified soil bacterium
1.1.3.23 65
-
 not stable above unidentified soil bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.23 1.67
-
 thiamine thiamine acetate formed unidentified soil bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.23 8 9
-
 unidentified soil bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.23 FAD
-
 unidentified soil bacterium