EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.15 | EDTA | slight activation for short and long chain oxidases | Sus scrofa |
EC Number | General Stability | Organism |
---|---|---|
1.1.3.15 | long chain oxidase stable at -20°C, short chain oxidase stable at -20°C for several months | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.15 | 2-Hydroxybutyrate | long chain oxidase, 13-17% inhibition at 17 mM | Sus scrofa | |
1.1.3.15 | 4-chloromercuribenzoate | long chain oxidase, 29-42% inhibition at 0.001 mM, short chain oxidase: 70-75% inhibition at 0.001 mM | Sus scrofa | |
1.1.3.15 | Atebrin | long chain oxidase, 72-76% inhibition at 1 mM, short chain oxidase: 68-76% inhibition at 1 mM | Sus scrofa | |
1.1.3.15 | Cu2+ | short chain oxidase: 86% inhibition at 0.1 mM | Sus scrofa | |
1.1.3.15 | DL-beta-Phenyllactate | short chain oxidase: 10% inhibition of glycolate oxidation, 81% inhibition of L-2-hydroxisocaproate oxidation at 10 mM | Sus scrofa | |
1.1.3.15 | DL-Lipoate | long chain oxidase, 35% inhibition at 0.24 mM, short chain oxidase: 46-52% inhibition at 0.01 mM | Sus scrofa | |
1.1.3.15 | iodoacetate | long chain oxidase, 31-36% inhibition at 0.1 mM, short chain oxidase: no inhibition | Sus scrofa | |
1.1.3.15 | L-leucine | 9-12% inhibition at 33 mM | Sus scrofa | |
1.1.3.15 | L-phenylalanine | long chain oxidase, 43% inhibition at 33 mM | Sus scrofa | |
1.1.3.15 | o-Iodosobenzoate | long chain oxidase, 95-100% inhibition at 0.1 mM, short chain oxidase: no inhibition | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.15 | 0.31 | - |
glycolate | short chain oxidase | Sus scrofa | |
1.1.3.15 | 0.68 | - |
L-2-Hydroxyisocaproate | short chain oxidase | Sus scrofa | |
1.1.3.15 | 0.68 | - |
L-2-Hydroxyisocaproate | short chain oxidase, at pH 6.0 and 37°C | Sus scrofa | |
1.1.3.15 | 2.2 | - |
L-beta-Phenyllactate | long chain oxidase | Sus scrofa | |
1.1.3.15 | 2.4 | - |
L-2-hydroxy-beta-methylvalerate | long chain oxidase | Sus scrofa | |
1.1.3.15 | 2.5 | - |
L-2-Hydroxyisocaproate | long chain oxidase | Sus scrofa | |
1.1.3.15 | 2.5 | - |
L-2-Hydroxyisocaproate | long chain oxidase, at pH 6.0 and 37°C | Sus scrofa | |
1.1.3.15 | 16 | - |
L-lactate | short chain oxidase | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.3.15 | microsome | - |
Sus scrofa | - |
- |
1.1.3.15 | mitochondrion | - |
Sus scrofa | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.15 | Sus scrofa | - |
pig | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.15 | to homogeneity, chromatography techniques, 2 distinct forms | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.15 | kidney | two forms: long chain oxidase, short chain oxidase | Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.15 | DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, low activity for short chain oxidase | Sus scrofa | 2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol | low activity for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol | low activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxocaproate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, traces of activity for short chain oxidase | Sus scrofa | 2-oxodecanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 2-oxooctanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol | low activity for short chain oxidase, moderate activity for long chain oxidase | Sus scrofa | 2-oxovalerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-3-indolelactate + 2,6-dichlorophenolindophenol | good substrate for long chain oxidase, no activity for short chain oxidase | Sus scrofa | 3-indolepyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-beta-phenyllactate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-mandelate + 2,6-dichlorophenolindophenol | low activity | Sus scrofa | oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | (4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | glycolate + 2,6-dichlorophenolindophenol | highest activity for short chain oxidase, no activity for long chain oxidase | Sus scrofa | glyoxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol | no activity for short chain oxidase | Sus scrofa | 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | L-2-hydroxyisocaproate + O2 | 100% activity with long chain oxidase, 32% activity with short chain oxidase | Sus scrofa | 4-methyl-2-oxopentanoate + H2O2 | - |
r | |
1.1.3.15 | L-lactate + 2,6-dichlorophenolindophenol | very low activity | Sus scrofa | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.3.15 | L-phenyllactate + 2,6-dichlorophenolindophenol | - |
Sus scrofa | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.15 | 7.7 | - |
short chain oxidase | Sus scrofa |
1.1.3.15 | 7.9 | - |
long chain oxidase | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.15 | FMN | - |
Sus scrofa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.15 | 2.5 | - |
Phenyllactate | competitive | Sus scrofa |