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Literature summary extracted from

  • Robinson, J.C.; Keay, L.; Molinari, R.; Sizer, I.W.
    L-alpha-Hydroxy acid oxidases of hog renal cortex (1962), J. Biol. Chem., 237, 2001-2010.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.15 EDTA slight activation for short and long chain oxidases Sus scrofa

General Stability

EC Number General Stability Organism
1.1.3.15 long chain oxidase stable at -20°C, short chain oxidase stable at -20°C for several months Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 2-Hydroxybutyrate long chain oxidase, 13-17% inhibition at 17 mM Sus scrofa
1.1.3.15 4-chloromercuribenzoate long chain oxidase, 29-42% inhibition at 0.001 mM, short chain oxidase: 70-75% inhibition at 0.001 mM Sus scrofa
1.1.3.15 Atebrin long chain oxidase, 72-76% inhibition at 1 mM, short chain oxidase: 68-76% inhibition at 1 mM Sus scrofa
1.1.3.15 Cu2+ short chain oxidase: 86% inhibition at 0.1 mM Sus scrofa
1.1.3.15 DL-beta-Phenyllactate short chain oxidase: 10% inhibition of glycolate oxidation, 81% inhibition of L-2-hydroxisocaproate oxidation at 10 mM Sus scrofa
1.1.3.15 DL-Lipoate long chain oxidase, 35% inhibition at 0.24 mM, short chain oxidase: 46-52% inhibition at 0.01 mM Sus scrofa
1.1.3.15 iodoacetate long chain oxidase, 31-36% inhibition at 0.1 mM, short chain oxidase: no inhibition Sus scrofa
1.1.3.15 L-leucine 9-12% inhibition at 33 mM Sus scrofa
1.1.3.15 L-phenylalanine long chain oxidase, 43% inhibition at 33 mM Sus scrofa
1.1.3.15 o-Iodosobenzoate long chain oxidase, 95-100% inhibition at 0.1 mM, short chain oxidase: no inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.31
-
glycolate short chain oxidase Sus scrofa
1.1.3.15 0.68
-
L-2-Hydroxyisocaproate short chain oxidase Sus scrofa
1.1.3.15 0.68
-
L-2-Hydroxyisocaproate short chain oxidase, at pH 6.0 and 37°C Sus scrofa
1.1.3.15 2.2
-
L-beta-Phenyllactate long chain oxidase Sus scrofa
1.1.3.15 2.4
-
L-2-hydroxy-beta-methylvalerate long chain oxidase Sus scrofa
1.1.3.15 2.5
-
L-2-Hydroxyisocaproate long chain oxidase Sus scrofa
1.1.3.15 2.5
-
L-2-Hydroxyisocaproate long chain oxidase, at pH 6.0 and 37°C Sus scrofa
1.1.3.15 16
-
L-lactate short chain oxidase Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.15 microsome
-
Sus scrofa
-
-
1.1.3.15 mitochondrion
-
Sus scrofa 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Sus scrofa
-
pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 to homogeneity, chromatography techniques, 2 distinct forms Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 kidney two forms: long chain oxidase, short chain oxidase Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, low activity for short chain oxidase Sus scrofa 2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol low activity for long chain oxidase, no activity for short chain oxidase Sus scrofa 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol low activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxocaproate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, traces of activity for short chain oxidase Sus scrofa 2-oxodecanoate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol no activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, no activity for short chain oxidase Sus scrofa 2-oxooctanoate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol low activity for short chain oxidase, moderate activity for long chain oxidase Sus scrofa 2-oxovalerate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-3-indolelactate + 2,6-dichlorophenolindophenol good substrate for long chain oxidase, no activity for short chain oxidase Sus scrofa 3-indolepyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-beta-phenyllactate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-mandelate + 2,6-dichlorophenolindophenol low activity Sus scrofa oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa (4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 glycolate + 2,6-dichlorophenolindophenol highest activity for short chain oxidase, no activity for long chain oxidase Sus scrofa glyoxylate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol no activity for short chain oxidase Sus scrofa 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 L-2-hydroxyisocaproate + O2 100% activity with long chain oxidase, 32% activity with short chain oxidase Sus scrofa 4-methyl-2-oxopentanoate + H2O2
-
r
1.1.3.15 L-lactate + 2,6-dichlorophenolindophenol very low activity Sus scrofa pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.15 L-phenyllactate + 2,6-dichlorophenolindophenol
-
Sus scrofa phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7.7
-
short chain oxidase Sus scrofa
1.1.3.15 7.9
-
long chain oxidase Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.15 2.5
-
Phenyllactate competitive Sus scrofa