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Literature summary extracted from

  • Ushijima, Y.
    Identity of aliphatic L-alpha-hydroxyacid oxidase and glycolate oxidase from rat liver (1973), Arch. Biochem. Biophys., 155, 361-367.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 4-chloromercuribenzoate strong inhibition at 0.01 mM Rattus sp.
1.1.3.15 8-hydroxyquinoline 5-17% inhibition at 1 mM Rattus sp.
1.1.3.15 Cu2+ nearly complete inhibition at 0.025 mM Rattus sp.
1.1.3.15 cysteine 28-38% inhibition of glycolate oxidation at 1 mM Rattus sp.
1.1.3.15 diethyldithiocarbamate competitive inhibition Rattus sp.
1.1.3.15 diphenylglycolic acid 11-17% inhibition at 52 mM Rattus sp.
1.1.3.15 hydoxylamine 66-67% inhibition at 5 mM Rattus sp.
1.1.3.15 KCN mixed-type inhibitor Rattus sp.
1.1.3.15 o-Iodosobenzoate 46-62% inhibition at 0.1 mM Rattus sp.
1.1.3.15 o-phenanthroline 5-23% inhibition at 1 mM Rattus sp.
1.1.3.15 oxalate 18% inhibition of glycolate oxidation, 71% inhibition of glyoxylate oxidation at 0.03 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.22
-
 glycolate cosubstrates: 2,4-dinitrophenyl hydrazone Rattus sp.
1.1.3.15 0.24
-
 glycolate cosubstrate: O2 Rattus sp.
1.1.3.15 1.24
-
 L-2-Hydroxyisocaproate cosubstrate: 2,4-dinitrophenyl hydrazone Rattus sp.
1.1.3.15 1.26
-
 L-2-Hydroxyisocaproate cosubstrate: O2 Rattus sp.
1.1.3.15 1.41
-
 glyoxylate cosubstrate: O2 Rattus sp.
1.1.3.15 1.78
-
 glyoxylate cosubstrate: 2,4-dinitrophenyl hydrazone Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.15 peroxisome
-
 Rattus sp. 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 D-lactate + O2 Rattus sp. traces of activity pyruvate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxybutyrate + O2 Rattus sp.
-
 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyisovalerate + O2 Rattus sp. low activity 2-oxoisovalerate + H2O2
-
 ?
1.1.3.15 glycolate + O2 Rattus sp.
-
 glyoxylate + H2O2
-
 ?
1.1.3.15 glyoxylate + O2 Rattus sp. 40% of the activity compared to glycolate ? + H2O2
-
 ?
1.1.3.15 L-2-hydroxyisocaproate + O2 Rattus sp.
-
 2-oxoisocaproate + H2O2
-
 ?
1.1.3.15 L-lactate + O2 Rattus sp. very low activity pyruvate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 partial Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 0.28
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 D-lactate + O2 traces of activity Rattus sp. pyruvate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxybutyrate + 2,4-dinitrophenylhydrazine low activity Rattus sp. 2-oxobutyrate 2,4-dinitrophenylhydrazone + ?
-
 ?
1.1.3.15 DL-2-hydroxybutyrate + O2
-
 Rattus sp. 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyisovalerate + 2,4-dinitrophenylhydrazine very low activity Rattus sp. 2-oxoisovalerate 2,4-dinitrophenylhydrazone + ?
-
 ?
1.1.3.15 DL-2-hydroxyisovalerate + O2 low activity Rattus sp. 2-oxoisovalerate + H2O2
-
 ?
1.1.3.15 glycolate + 2,4-dinitrophenylhydrazine best substrate tested Rattus sp. glyoxylate 2,4-dinitrophenylhydrazone + ?
-
 ?
1.1.3.15 glycolate + O2
-
 Rattus sp. glyoxylate + H2O2
-
 ?
1.1.3.15 glyoxylate + O2 40% of the activity compared to glycolate Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-2-hydroxyisocaproate + 2,4-dinitrophenylhydrazine
-
 Rattus sp. 2-oxoisocaproate 2,4-dinitrophenylhydrazone + ?
-
 ?
1.1.3.15 L-2-hydroxyisocaproate + O2
-
 Rattus sp. 2-oxoisocaproate + H2O2
-
 ?
1.1.3.15 L-lactate + 2,4-dinitrophenylhydrazine very low activity Rattus sp. pyruvate 2,4-dinitrophenylhydrazone + ?
-
 ?
1.1.3.15 L-lactate + O2 very low activity Rattus sp. pyruvate + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 8.5
-
-
 Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.15 0.27 0.39 diethyldithiocarbamate competitive inhibition Rattus sp.
1.1.3.15 0.52 0.65 KCN mixed-type inhibition Rattus sp.