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Literature summary extracted from

  • Tsukatani, T.; Matsumoto, K.
    Quantification of L-tartrate in wine by stopped-flow injection analysis using immobilized D-malate dehydrogenase and fluorescence detection (2000), Anal. Sci., 16, 265-268.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.93 analysis quantification of L-tartrate in wine by a stopped-flow injection system with an immobilized enzyme reactor and fluorescence detection, the enzyme is immobilized on aminopropyl-controlled pore glass beads with glutaraldehyde Escherichia coli

General Stability

EC Number General Stability Organism
1.1.1.93 the enzyme reactor is stored at 5°C in 0.1 M phosphate buffer, pH 8.0, 10 mM dithiothreitol, when not in use, 40% loss of activity after 1 week, then activity maintains a constant value for 1 month Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.93 Mg2+ required Escherichia coli
1.1.1.93 Mg2+ optimal concentration: 50 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.93 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.93 L-tartrate + NAD+
-
 Escherichia coli oxaloglycolate + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.93 9.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.93 NAD+
-
 Escherichia coli