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Literature summary extracted from

  • Estevez, M.P.; Legaz, E.; Olmeda, L.; Perez, F.J.; Vicente, C.
    Purification and properties of a new enzyme from Evernia prunastri, which reduces L-usnic acid (1981), Z. Naturforsch. C, 36, 35-39.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.199 thermolabile Evernia prunastri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.199 450000
-
 gel filtration Evernia prunastri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.199 L-usnic acid + NADH Evernia prunastri
-
 NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.199 Evernia prunastri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.199
-
 Evernia prunastri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.199 thallus
-
 Evernia prunastri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.199 L-usnic acid + NADH
-
 Evernia prunastri NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.199 30
-
-
 Evernia prunastri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.199 22 37
-
 Evernia prunastri
1.1.1.199 22
-
 about 30% of maximal activity Evernia prunastri
1.1.1.199 37
-
 about 35% of maximal activity Evernia prunastri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.199 8
-
-
 Evernia prunastri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.199 6.8 9.2
-
 Evernia prunastri
1.1.1.199 6.8
-
 about 20% of maximal activity Evernia prunastri
1.1.1.199 9.2
-
 about 50% of maximal activity Evernia prunastri

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.199 NADH strict requirement for NADH, not replaceable by NADPH Evernia prunastri