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Literature summary extracted from

  • Singh, S.P.; Rogers, P.J.
    Isolation and characterization of mannitol-1-phosphate dehydrogenase from Brochothrix thermosphacta (1993), J. Gen. Appl. Microbiol., 39, 327-337.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 ADP competitive inhibition Brochothrix thermosphacta
1.1.1.17 ATP competitive inhibition Brochothrix thermosphacta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 0.03
-
 NADH higher affinity for NADH Brochothrix thermosphacta
1.1.1.17 0.05
-
 NAD+
-
 Brochothrix thermosphacta
1.1.1.17 0.05
-
 D-mannitol 1-phosphate cosubstrate: NAD+ Brochothrix thermosphacta
1.1.1.17 0.25
-
 D-fructose 6-phosphate cosubstrate: NADH Brochothrix thermosphacta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
 Brochothrix thermosphacta 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 50000
-
 gel filtration Brochothrix thermosphacta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Brochothrix thermosphacta
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Brochothrix thermosphacta
-
 formerly Microbacterium thermosphactum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 to homogeneity, chromatography techniques Brochothrix thermosphacta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture inducible by D-mannitol Brochothrix thermosphacta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 0.09
-
 crude extract, D-mannitol 1-phosphate oxidation Brochothrix thermosphacta
1.1.1.17 18
-
 purified enzyme, D-fructose 6-phosphate reduction Brochothrix thermosphacta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Brochothrix thermosphacta D-fructose 6-phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.17 monomer 1 * 50000, SDS-PAGE Brochothrix thermosphacta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 6 7 oxidation of D-mannitol 1-phosphate Brochothrix thermosphacta
1.1.1.17 9.5 10 reduction of D-fructose 6-phosphate Brochothrix thermosphacta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ higher affinity for NADH Brochothrix thermosphacta
1.1.1.17 NADH higher affinity than for NAD+ Brochothrix thermosphacta

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.17 0.7
-
 ADP more potent inhibitor than ATP Brochothrix thermosphacta
1.1.1.17 1.6
-
 ATP
-
 Brochothrix thermosphacta