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Literature summary extracted from
- Bovine adrenal 3beta-hydroxysteroid dehydrogenase (E.C. 1.1.1.145)/5-ene-4-ene isomerase (E.C. 5.3.3.1): characterization and its inhibition by isoflavones (1999), J. Steroid Biochem. Mol. Biol., 71, 191-202.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.145 | 17beta-estradiol | - |
Bos taurus |  |
| 1.1.1.145 | apigenin | - |
Bos taurus | |
| 1.1.1.145 | Biochanin A | - |
Bos taurus | |
| 1.1.1.145 | daidzein | - |
Bos taurus | |
| 1.1.1.145 | daidzein | - |
Cricetinae | |
| 1.1.1.145 | formononetin | - |
Bos taurus | |
| 1.1.1.145 | genistein | - |
Bos taurus | |
| 1.1.1.145 | prunetin | - |
Bos taurus | |
| 1.1.1.145 | puerarin | - |
Bos taurus | |
| 5.3.3.1 | 17beta-estradiol | IC50: 0.0003 mM | Bos taurus | |
| 5.3.3.1 | Biochanin A | IC50: 0.0004 mM | Bos taurus | |
| 5.3.3.1 | daidzein | IC50: 0.0038 mM | Bos taurus | |
| 5.3.3.1 | formononetin | IC50: 0.004 mM | Bos taurus | |
| 5.3.3.1 | genistein | IC50: 0.0023 mM | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.145 | 0.001 | - |
dehydroepiandrosterone | - |
Bos taurus | |
| 1.1.1.145 | 0.001 | - |
allopregnanolone | - |
Bos taurus | |
| 1.1.1.145 | 0.001 | - |
androstenediol | - |
Bos taurus | |
| 1.1.1.145 | 0.002 | - |
pregnenolone | - |
Bos taurus | |
| 5.3.3.1 | 0.01 | - |
DELTA5-pregnen-3,20-dione | 25°C, pH 7.5 | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.145 | microsome | - |
Bos taurus | - |
- |
| 5.3.3.1 | microsome | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.145 | pregnenolone + NAD(P)+ | Bos taurus | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
| 1.1.1.145 | pregnenolone + NAD(P)+ | Cricetinae | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.145 | Bos taurus | - |
- |
- |
| 1.1.1.145 | Cricetinae | - |
- |
- |
| 5.3.3.1 | Bos taurus | - |
3beta-hydroxy-5-ene-steroid dehydrogenase/steroid DELTA5-DELTA4-ene isomerase enzyme complex | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.145 | - |
Bos taurus |
| 5.3.3.1 | 3beta-hydroxy-5-ene-steroid dehydrogenase/steroid DELTA5-DELTA4-ene isomerase enzyme complex | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.145 | 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Bos taurus | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
| 1.1.1.145 | 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Cricetinae | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
| 1.1.1.145 | 5alpha-pregnan-3beta-ol-20-one + NAD+ | - |
Bos taurus | 5alpha-pregnan-3,20-dione + NADH | - |
? | |
| 1.1.1.145 | allopregnanolone + NAD+ | - |
Bos taurus | ? | - |
r | |
| 1.1.1.145 | androstenediol + NAD+ | - |
Bos taurus | testosterone + NADH + H+ | - |
r | |
| 1.1.1.145 | dehydroepiandrosterone + NAD+ | - |
Bos taurus | androst-5-en-3,17-dione + NADH + H+ | - |
? | |
| 1.1.1.145 | pregnenolone + NAD(P)+ | - |
Bos taurus | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
| 1.1.1.145 | pregnenolone + NAD(P)+ | - |
Cricetinae | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
| 5.3.3.1 | DELTA5-pregnen-3,20-dione | - |
Bos taurus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.3.1 | 3beta-HSD/isomerase | - |
Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.145 | 0.0233 | - |
allopregnanolone | - |
Bos taurus | |
| 1.1.1.145 | 0.0333 | - |
dehydroepiandrosterone | - |
Bos taurus | |
| 1.1.1.145 | 0.0333 | - |
androstenediol | - |
Bos taurus | |
| 1.1.1.145 | 0.04 | - |
pregnenolone | - |
Bos taurus | |
| 5.3.3.1 | 0.303 | - |
DELTA5-pregnen-3,20-dione | 25°C, pH 7.5 | Bos taurus | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.145 | NAD+ | - |
Bos taurus | |
| 1.1.1.145 | NAD+ | - |
Cricetinae | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.1 | 0.0003 | - |
IC50: 0.0003 mM | Bos taurus | 17beta-estradiol | |
| 5.3.3.1 | 0.0004 | - |
IC50: 0.0004 mM | Bos taurus | Biochanin A | |
| 5.3.3.1 | 0.0023 | - |
IC50: 0.0023 mM | Bos taurus | genistein | |
| 5.3.3.1 | 0.0038 | - |
IC50: 0.0038 mM | Bos taurus | daidzein | |
| 5.3.3.1 | 0.004 | - |
IC50: 0.004 mM | Bos taurus | formononetin | |
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Release 2023.1 (January 2023)
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