BRENDA - Enzyme Database

Acetyl-coenzyme A carboxylase from avocado (Persea americana) plastids and spinach (Spinacia oleracea) chloroplasts

Mohan, S.B.; Kekwick, R.G.O.; Biochem. J. 187, 667-676 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
citrate
activates; maximal activation at 3.0 mM, 2fold
Persea americana
6.4.1.2
isocitrate
maximal activation at 3 mM
Persea americana
6.4.1.2
isocitrate
maximal activation at 3 mM
Spinacia oleracea
General Stability
EC Number
General Stability
Organism
6.4.1.2
bovine serum albumin, HCO3-, citrate, and glycerol required for stabilization
Persea americana
6.4.1.2
requires bovine serum albumin, HCO3-, citrate, and glycerol for stabilization
Spinacia oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
citrate
above 4.0 mM
Persea americana
6.4.1.2
CuSO4
-
Persea americana
6.4.1.2
diisopropyl phosphorofluoridate
-
Persea americana
6.4.1.2
iodoacetamide
-
Persea americana
6.4.1.2
Phenyl chloromercuribenzoate
-
Persea americana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
additional information
-
additional information
effect of citrate or isocitrate on Km-values
Persea americana
6.4.1.2
additional information
-
additional information
effect of citrate or isocitrate on Km-values
Spinacia oleracea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.2
chloroplast
lamellae
Persea americana
9507
-
6.4.1.2
chloroplast
-
Spinacia oleracea
9507
-
6.4.1.2
plastid
-
Persea americana
9536
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Mg2+
Mg2+ or Mn2+ essential for activity
Persea americana
6.4.1.2
Mg2+
Mg2+ or Mn2+ essential for activity
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
12000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
47000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
57000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
72000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
650000
-
gel filtration
Persea americana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.4.1.2
Persea americana
-
-
-
6.4.1.2
Spinacia oleracea
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.4.1.2
mesocarp
-
Persea americana
-
Storage Stability
EC Number
Storage Stability
Organism
6.4.1.2
-20C, 0.1 M potassium phosphate, 0.5 mM EDTA, pH 7.3, 1 mM DTT, 10 mM trisodium citrate, 15 mM NaHCO3, 0.1% bovine serum albumin, 15% v/v glycerol, stable for 2 months
Persea americana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37594
Spinacia oleracea
ADP + phosphate + malonyl-CoA
-
37594
Spinacia oleracea
-
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37594
Persea americana
ADP + phosphate + malonyl-CoA
-
37594
Persea americana
-
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.2
?
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
6.4.1.2
37
-
-
Persea americana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
7.5
-
-
Persea americana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
citrate
activates; maximal activation at 3.0 mM, 2fold
Persea americana
6.4.1.2
isocitrate
maximal activation at 3 mM
Persea americana
6.4.1.2
isocitrate
maximal activation at 3 mM
Spinacia oleracea
General Stability (protein specific)
EC Number
General Stability
Organism
6.4.1.2
bovine serum albumin, HCO3-, citrate, and glycerol required for stabilization
Persea americana
6.4.1.2
requires bovine serum albumin, HCO3-, citrate, and glycerol for stabilization
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
citrate
above 4.0 mM
Persea americana
6.4.1.2
CuSO4
-
Persea americana
6.4.1.2
diisopropyl phosphorofluoridate
-
Persea americana
6.4.1.2
iodoacetamide
-
Persea americana
6.4.1.2
Phenyl chloromercuribenzoate
-
Persea americana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
additional information
-
additional information
effect of citrate or isocitrate on Km-values
Persea americana
6.4.1.2
additional information
-
additional information
effect of citrate or isocitrate on Km-values
Spinacia oleracea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.2
chloroplast
lamellae
Persea americana
9507
-
6.4.1.2
chloroplast
-
Spinacia oleracea
9507
-
6.4.1.2
plastid
-
Persea americana
9536
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Mg2+
Mg2+ or Mn2+ essential for activity
Persea americana
6.4.1.2
Mg2+
Mg2+ or Mn2+ essential for activity
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
12000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
47000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
57000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
72000
-
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
6.4.1.2
650000
-
gel filtration
Persea americana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.4.1.2
mesocarp
-
Persea americana
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.4.1.2
-20C, 0.1 M potassium phosphate, 0.5 mM EDTA, pH 7.3, 1 mM DTT, 10 mM trisodium citrate, 15 mM NaHCO3, 0.1% bovine serum albumin, 15% v/v glycerol, stable for 2 months
Persea americana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37594
Spinacia oleracea
ADP + phosphate + malonyl-CoA
-
37594
Spinacia oleracea
-
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37594
Persea americana
ADP + phosphate + malonyl-CoA
-
37594
Persea americana
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.2
?
x * 12000 + x * 47000 + x * 57000 + x * 72000, SDS-PAGE + 1% mercaptoethanol
Persea americana
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
6.4.1.2
37
-
-
Persea americana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
7.5
-
-
Persea americana