BRENDA - Enzyme Database

Glutamine synthetase from Pseudomonas syringae pv. tabaci: properties and inhibition by tabtoxinine-beta-lactam

Thomas, M.D.; Durbin, R.D.; J. Gen. Microbiol. 131, 1061-1067 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.1.2
ATP
-
Pseudomonas syringae
6.3.1.2
L-Glu
50-100 mM, substrate inhibition
Pseudomonas syringae
6.3.1.2
Mg2+
inhibits gamma-glutamyl transferase activity
Pseudomonas syringae
6.3.1.2
NH4Cl
substrate inhibition at high concentrations
Pseudomonas syringae
6.3.1.2
Tabtoxinine-beta-lactam
-
Pseudomonas syringae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.1.2
60000
-
x * 60000, SDS-PAGE
Pseudomonas syringae
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.3.1.2
Pseudomonas syringae
-
pv. Tabaci
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.1.2
-
Pseudomonas syringae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.1.2
41.6
-
gamma-glutamylhydroxamate assay
Pseudomonas syringae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.3.1.2
ATP + L-Glu + NH4+
-
37500
Pseudomonas syringae
ADP + phosphate + L-Gln
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.1.2
?
x * 60000, SDS-PAGE
Pseudomonas syringae
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.1.2
60
-
stable for 1 h
Pseudomonas syringae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.1.2
ATP
-
Pseudomonas syringae
6.3.1.2
L-Glu
50-100 mM, substrate inhibition
Pseudomonas syringae
6.3.1.2
Mg2+
inhibits gamma-glutamyl transferase activity
Pseudomonas syringae
6.3.1.2
NH4Cl
substrate inhibition at high concentrations
Pseudomonas syringae
6.3.1.2
Tabtoxinine-beta-lactam
-
Pseudomonas syringae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.1.2
60000
-
x * 60000, SDS-PAGE
Pseudomonas syringae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.1.2
-
Pseudomonas syringae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.1.2
41.6
-
gamma-glutamylhydroxamate assay
Pseudomonas syringae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.3.1.2
ATP + L-Glu + NH4+
-
37500
Pseudomonas syringae
ADP + phosphate + L-Gln
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.1.2
?
x * 60000, SDS-PAGE
Pseudomonas syringae
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.1.2
60
-
stable for 1 h
Pseudomonas syringae