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Literature summary extracted from

  • Murata, K.; Saikusa, T.; Watanabe, K.; Inoue, Y.; Fukuda, Y.; Shimosaka, M.; Kimura, A.
    Complete purification of yeast glyoxalase I and its activation by ferrous ions and polyamines (1986), Agric. Biol. Chem., 50, 2381-2383.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.5 polyamines activate Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 EDTA
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Ca2+ restores activity after EDTA treatment Saccharomyces cerevisiae
4.4.1.5 Fe2+ activates Saccharomyces cerevisiae
4.4.1.5 Mg2+ restores activity after EDTA treatment Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 33000
-
 gel giltration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal Saccharomyces cerevisiae detoxification of methylglyoxal ?
-
 ?
4.4.1.5 glutathione + methylglyoxal Saccharomyces cerevisiae DKD-5D-H detoxification of methylglyoxal ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Saccharomyces cerevisiae
-
 DKD-5D-H
-
4.4.1.5 Saccharomyces cerevisiae DKD-5D-H
-
 DKD-5D-H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 963
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Saccharomyces cerevisiae S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal
-
 Saccharomyces cerevisiae DKD-5D-H S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal detoxification of methylglyoxal Saccharomyces cerevisiae ?
-
 ?
4.4.1.5 glutathione + methylglyoxal detoxification of methylglyoxal Saccharomyces cerevisiae DKD-5D-H ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 monomer 1 * 33000, SDS-PAGE Saccharomyces cerevisiae