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Literature summary extracted from

  • Chen, C.C.; Somberg, E.W.
    Purification and characterization of histidyl-transfer RNA synthetase from Neurospora crassa (1980), Biochim. Biophys. Acta, 613, 514-525.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.21 bovine serum albumin required to prevent inactivation Neurospora crassa
6.1.1.21 loss of activity after freezing and thawing Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 Argininosuccinic acid poor inhibitor Neurospora crassa
6.1.1.21 Imidazoleglycerol phosphate
-
 Neurospora crassa
6.1.1.21 Indoleglycerol phosphate
-
 Neurospora crassa
6.1.1.21 L-histidinol
-
 Neurospora crassa
6.1.1.21 ornithine poor inhibitor Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.21 0.00012
-
 tRNAHis aminoacylation Neurospora crassa
6.1.1.21 0.0058
-
 His aminoacylation Neurospora crassa
6.1.1.21 0.59
-
 ATP aminoacylation Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.21 Mg2+ optimal concentration is 20 mM Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 62500
-
 2 * 62500, SDS-PAGE Neurospora crassa
6.1.1.21 125000
-
 gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21
-
 Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.21 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.21 additional information
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
6.1.1.21 -20°C, 10 mM Hepes, pH 7.4, 1 mM magnesium acetate, 1 mM KCl, 0.5 mM DTT, 0.1 mM phenylmethylsulfonyl fluoride, 50% glycerol, stable for 6 months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Neurospora crassa AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 dimer 2 * 62500, SDS-PAGE Neurospora crassa