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Literature summary extracted from

  • Kobayashi, T.; Koike, K.; Yoshimatsu, T.; Higaki, N.; Suzumatsu, A.; Ozawa, T.; Hatada, Y.; Ito, S.
    Purification and properties of a low-molecular-weight, high-alkaline pectate lyase from an alkaliphilic strain of Bacillus (1999), Biosci. Biotechnol. Biochem., 63, 65-72.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 2,3-Butanedione
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 2,4,6-Trinitrobenzenesulfonate 1 mM, 73% inhibition Bacillus sp. (in: Bacteria)
4.2.2.2 Hg2+
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 N-bromosuccinimide 0.1 mM, 32% inhibition Bacillus sp. (in: Bacteria)
4.2.2.2 Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km for polygalacturonic acid: 16% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 20300
-
 analytical ultracentrifugation Bacillus sp. (in: Bacteria)
4.2.2.2 26000
-
 x * 26000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus sp. (in: Bacteria)
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) KSM-P15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 additional information protopectinase-like activity on cotton fibers Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.2 additional information protopectinase-like activity on cotton fibers Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
4.2.2.2 polygalacturonic acid random attack Bacillus sp. (in: Bacteria) unsaturated oligogalacturonate a mixture of 4,5-unsaturated oligogalacturonides, approximately molar ratios of 12:74:14 for monomer, dimer, and trimer, respectively ?
4.2.2.2 polygalacturonic acid random attack Bacillus sp. (in: Bacteria) KSM-P15 unsaturated oligogalacturonate a mixture of 4,5-unsaturated oligogalacturonides, approximately molar ratios of 12:74:14 for monomer, dimer, and trimer, respectively ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 26000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 50 55
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 30 60 30°C: about 25% of maximal activity, 60°C: about 40% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 additional information
-
 Ca2+ slightly enhances thermal stability Bacillus sp. (in: Bacteria)
4.2.2.2 50
-
 30 min, stable up to Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 9.5 11.5 about 30% of maximal activity at pH 9.5 and at pH 11.5 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 5 11 30°C, 1 h, with or without CaCl2, stable Bacillus sp. (in: Bacteria)