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Literature summary extracted from

  • Miyazaki, Y.
    Purification and characterization of endo-pectate lyase from Bacillus macerans (1991), Agric. Biol. Chem., 55, 25-30.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Ba2+
-
 Paenibacillus macerans
4.2.2.2 EDTA
-
 Paenibacillus macerans
4.2.2.2 Zn2+
-
 Paenibacillus macerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km for polypectate: 0.11% Paenibacillus macerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular
-
 Paenibacillus macerans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ activates Paenibacillus macerans
4.2.2.2 Ca2+ enhances activity 5fold Paenibacillus macerans
4.2.2.2 Mn2+ 1 mM, enhances activity 6fold Paenibacillus macerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 35000
-
 1 * 35000, SDS-PAGE Paenibacillus macerans
4.2.2.2 45000
-
 non-denaturing PAGE Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Paenibacillus macerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Paenibacillus macerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 additional information
-
-
 Paenibacillus macerans

Storage Stability

EC Number Storage Stability Organism
4.2.2.2 10°C, 100 mM ammonium chloride buffer, pH 9.0, stable for 1 month, 70% loss of activity in 20 mM ammonium chloride buffer, pH 9.0, after 24 h Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin with 4.3% methoxyl groups Paenibacillus macerans ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Paenibacillus macerans unsaturated oligogalacturonate
-
 ?
4.2.2.2 polypectate
-
 Paenibacillus macerans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 monomer 1 * 35000, SDS-PAGE Paenibacillus macerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 60
-
-
 Paenibacillus macerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 40 70 40°C: about 60% of maximal activity, 70°C: about 10% of maximal activity Paenibacillus macerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 37
-
 pH 6.5-9.6, 2 h, stable Paenibacillus macerans
4.2.2.2 50
-
 10 min, stable in absence of Ca2+ Paenibacillus macerans
4.2.2.2 55
-
 10 min, stable in presence of Ca2+ Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 9
-
 at 37°C Paenibacillus macerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 6.5 9.5 37°C, 2 h Paenibacillus macerans