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Literature summary extracted from

  • Kobayashi, T.; Hatada, Y.; Higaki, N.; Lusterio, D.D.; Ozawa, T.; Koike, K.; Kawai, S.; Ito, S.
    Enzymatic properties and deduced amino acid sequence of a high-alkaline pectate lyase from an alkaliphilic Bacillus isolate (1999), Biochim. Biophys. Acta, 1427, 145-154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 2,3-Butanedione
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 2-hydroxy-5-nitrophenyl bromide
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 Fe3+
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)
4.2.2.2 Mn2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)
4.2.2.2 Sr2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 33355
-
 x * 33355, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus sp. (in: Bacteria)
-
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) KSM-P7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) unsaturated oligogalacturonate
-
 ?
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) KSM-P7 unsaturated oligogalacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 33355, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 60 65
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 55
-
 stable up to, in absence of CaCl2 Bacillus sp. (in: Bacteria)
4.2.2.2 60
-
 Ca2+ slightly enhances thermostability up to 60°C Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 4 12 5°C, 24 h, stable Bacillus sp. (in: Bacteria)