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Literature summary extracted from

  • Suzuki, S.; Hirahara, T.; Horinouchi, S.; Beppu, T.
    Purification and properties of thermostable tryptophanase from an obligately symbiotic thermophile, Symbiobacterium thermophilum (1991), Agric. Biol. Chem., 55, 3059-3066.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.1 1.47
-
 L-Trp
-
 Symbiobacterium thermophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.1 K+ activates Symbiobacterium thermophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 46000
-
 4 * 46000, SDS-PAGE Symbiobacterium thermophilum
4.1.99.1 210000
-
 gel filtration Symbiobacterium thermophilum

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Symbiobacterium thermophilum
-
-
-
4.1.99.1 Symbiobacterium thermophilum T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1
-
 Symbiobacterium thermophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.1 7.6
-
-
 Symbiobacterium thermophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 5-hydroxy-L-Trp + H2O 6.7% of the activity with L-Trp Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 5-hydroxy-L-Trp + H2O 6.7% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
 ?
4.1.99.1 5-methyl-L-Trp + H2O 15.2% of the activity with L-Trp Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 5-methyl-L-Trp + H2O 15.2% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
 ?
4.1.99.1 Cys + H2O 18.9% of the activity with L-Trp Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 Cys + H2O L-Cys Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 Cys + H2O 18.9% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
 ?
4.1.99.1 Cys + H2O L-Cys Symbiobacterium thermophilum T ?
-
 ?
4.1.99.1 indole + pyruvate + NH4+
-
 Symbiobacterium thermophilum L-Trp + H2O
-
 ?
4.1.99.1 L-Trp + H2O
-
 Symbiobacterium thermophilum indole + pyruvate + NH4+
-
 ?
4.1.99.1 S-methyl-L-Cys + H2O 63.8% of the activity with L-Trp Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 S-methyl-L-Cys + H2O 63.8% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
 ?
4.1.99.1 Ser + H2O L-Ser Symbiobacterium thermophilum ?
-
 ?
4.1.99.1 Ser + H2O 5.3% of the activity with L-Trp Symbiobacterium thermophilum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.1 tetramer 4 * 46000, SDS-PAGE Symbiobacterium thermophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.1 70
-
-
 Symbiobacterium thermophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.99.1 65
-
 20 min stable Symbiobacterium thermophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.1 42
-
 L-Trp
-
 Symbiobacterium thermophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.1 7
-
-
 Symbiobacterium thermophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.99.1 6 10 25°C, 28 h, stable Symbiobacterium thermophilum