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Literature summary extracted from

  • Yoshida, H.; Kumagai, H.; Yamada, H.; Matsubara, H.
    The tryptophanase from Proteus rettgeri, improved purification and properties of crystalline holotryptophanase (1975), Biochim. Biophys. Acta, 391, 494-503.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.99.1
-
 Providencia rettgeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.1 guanidine hydrochloride
-
 Providencia rettgeri
4.1.99.1 SDS
-
 Providencia rettgeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.1 0.26
-
 L-Trp
-
 Providencia rettgeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 55000
-
 4 * 55000, SDS-PAGE Providencia rettgeri
4.1.99.1 222000
-
 ultracentrifugation and sedimentation measurement Providencia rettgeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.1 additional information Providencia rettgeri inducible enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Providencia rettgeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1
-
 Providencia rettgeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.1 9.01
-
-
 Providencia rettgeri

Storage Stability

EC Number Storage Stability Organism
4.1.99.1 5°C, stored as a suspension in 0.1 M potassium phosphate buffer, pH 8.0, containing 20% v/v glycerol, 0.1 mM pyridoxal 5'-phosphate, 10 mM mercaptoethanol and 60% saturated ammonium sulfate, stable for more than 5 months Providencia rettgeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-Trp + H2O
-
 Providencia rettgeri indole + pyruvate + NH4+
-
 ?
4.1.99.1 additional information inducible enzyme Providencia rettgeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.1 tetramer 4 * 55000, SDS-PAGE Providencia rettgeri

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.1 pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Providencia rettgeri