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Literature summary extracted from

  • Amagase, S.
    Digestive enzymes in insectivorous plants. III. Acid proteases in the genus Nepenthes and Drosera peltata (1972), J. Biochem., 72, 73-81.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.12 Peptides + H2O Drosera peltata preferential cleavage: -Asp, Asp-, Lys-, Ala- ?
-
 ?
3.4.23.12 Peptides + H2O Nepenthes sp. preferential cleavage: -Asp, Asp-, Lys-, Ala- ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.12 Drosera peltata
-
-
-
3.4.23.12 Nepenthes sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.23.12 Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg splits peptide bonds at carboxyl and amino sides of aspartic acid residue, at carboxyl side of alanine and probably carboxyl side of lysine Drosera peltata
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.12 LFNQDVDAAVR + H2O
-
 Nepenthes sp. LFNQDVD + AAVR
-
 ?
3.4.23.12 LQQKRWDEAA + H2O
-
 Nepenthes sp. LQQK + L-Arg-L-Trp + DEAA
-
 ?
3.4.23.12 NQDVDAAVRGIK + H2O
-
 Nepenthes sp. NQDV + L-Asp + AAVRGIK
-
 ?
3.4.23.12 Peptides + H2O preferential cleavage: -Asp, Asp-, Lys-, Ala- Drosera peltata ?
-
 ?
3.4.23.12 Peptides + H2O preferential cleavage: -Asp, Asp-, Lys-, Ala- Nepenthes sp. ?
-
 ?
3.4.23.12 TGTWDAYK + H2O
-
 Nepenthes sp. TGTWDA + YK
-
 ?
3.4.23.12 Thr-Ile-Asp-Glu + H2O
-
 Nepenthes sp. Thr-Ile-Asp + Glu
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.12 37
-
 assay at Drosera peltata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.12 40
-
 pH 3.0-9.0 Drosera peltata
3.4.23.12 60
-
 highest stability at pH 5 Drosera peltata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.12 3
-
-
 Drosera peltata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.12 1.7 3.2
-
 Drosera peltata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.12 3 9 40°C, highest stability at pH 3.0-9.0 Drosera peltata
3.4.23.12 5
-
 60°C, highest stability at pH 5 Drosera peltata