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Literature summary extracted from

  • Lamsal, M.; Agarwal, S.K.; Choudhury, S.D.; Khan, M.Y.
    Purification and tissue/species dependence of the specificity of buffalo kidney cathepsin B (1997), Indian J. Biochem. Biophys., 34, 461-469.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 2-mercaptoethanol activates Bubalus bubalis
3.4.22.1 cysteamine activates Bubalus bubalis
3.4.22.1 cysteine activates Bubalus bubalis
3.4.22.1 dithiothreitol activates Bubalus bubalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 antipain
-
 Bubalus bubalis
3.4.22.1 leupeptin
-
 Bubalus bubalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.00143 0.00217 hemoglobin
-
 Bubalus bubalis
3.4.22.1 0.09
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide
-
 Bubalus bubalis
3.4.22.1 0.17
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Bubalus bubalis
3.4.22.1 2.08
-
 benzoyl-DL-Arg-p-nitroanilide
-
 Bubalus bubalis
3.4.22.1 3.3
-
 benzoyl-DL-Arg-beta-naphthylamide
-
 Bubalus bubalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 25500 27000 gel filtration, SDS-PAGE Bubalus bubalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Bubalus bubalis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Bubalus bubalis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.1 side-chain modification glycoprotein Bubalus bubalis
3.4.22.1 side-chain modification 3.6% carbohydrate Bubalus bubalis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Bubalus bubalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 0.385
-
 benzoyl-DL-Arg-beta-naphthylamide as substrate Bubalus bubalis
3.4.22.1 0.851
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide as substrate Bubalus bubalis
3.4.22.1 1.538
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide as substrate Bubalus bubalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Bubalus bubalis benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O
-
 Bubalus bubalis benzoyl-DL-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Bubalus bubalis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
 Bubalus bubalis benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 Bovine serum albumin + H2O
-
 Bubalus bubalis Hydrolyzed bovine serum albumin
-
 ?
3.4.22.1 casein + H2O
-
 Bubalus bubalis hydrolyzed casein
-
 ?
3.4.22.1 Hemoglobin + H2O from goat and from buffalo Bubalus bubalis Hydrolyzed hemoglobin
-
 ?
3.4.22.1 Proteins + H2O
-
 Bubalus bubalis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 40
-
-
 Bubalus bubalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6.8
-
-
 Bubalus bubalis