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Literature summary extracted from

  • Masaki, T.; Nakamura, T.; Isono, M.; Soejima, M.
    A new proteolytic enzyme from Achromobacter lyticus M497-1 (1978), Agric. Biol. Chem., 42, 1443-1445.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 diisopropylfluorophosphate
-
 Achromobacter lyticus
3.4.21.50 Nalpha-p-tosyl-L-Lys-chloromethylketone
-
 Achromobacter lyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.50 0.07
-
 N-benzoyl-L-Lys p-nitroanilide protease I Achromobacter lyticus
3.4.21.50 0.08
-
 Lys-p-nitroanilide protease I Achromobacter lyticus
3.4.21.50 0.32
-
 benzoyl-Lys-NH2
-
 Achromobacter lyticus
3.4.21.50 20
-
 benzoyl-Arg ethyl ester
-
 Achromobacter lyticus
3.4.21.50 35.7
-
 N-tosyl-L-Arg methyl ester protease I Achromobacter lyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 27000
-
-
 Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Achromobacter lyticus
-
 M497-1
-
3.4.21.50 Achromobacter lyticus M497-1
-
 M497-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50
-
 Achromobacter lyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 benzoyl-Arg ethyl ester + H2O
-
 Achromobacter lyticus benzoyl-Arg + ethanol
-
 ?
3.4.21.50 benzoyl-Lys-NH2 + H2O
-
 Achromobacter lyticus benzoyl-Lys + NH3
-
 ?
3.4.21.50 Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus Lys + p-nitroaniline
-
 ?
3.4.21.50 Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 Lys + p-nitroaniline
-
 ?
3.4.21.50 additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus ?
-
 ?
3.4.21.50 additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus M497-1 ?
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
3.4.21.50 N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Arg + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Lys + methanol
-
 ?