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Literature summary extracted from

  • Grant, G.A.; Eisen, A.Z.; Bradshaw, R.A.
    Collagenolytic protease from fiddler crab (Uca pugilator) (1981), Methods Enzymol., 80, 722-734.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.32 0.044
-
 N-acetyl-L-Ala-L-Ala-L-Ala-p-nitroanilide
-
 Leptuca pugilator
3.4.21.32 0.1
-
 N-benzoyl-L-Arg-p-nitroanilide
-
 Leptuca pugilator
3.4.21.32 0.12
-
 N-benzoyl-L-Val-Gly-L-Arg-p-nitroanilide
-
 Leptuca pugilator
3.4.21.32 0.233
-
 Nalpha-tosyl-L-Arg methyl ester
-
 Leptuca pugilator
3.4.21.32 0.263
-
 N-benzoyl-L-Tyr-p-nitroanilide
-
 Leptuca pugilator
3.4.21.32 1.11
-
 N-benzoyl-L-Tyr ethyl ester
-
 Leptuca pugilator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.32 25000
-
 sedimentation-equilibrium centrifugation Leptuca pugilator

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.32 Leptuca pugilator
-
 fiddler crab
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.32 Collagen + H2O
-
 Leptuca pugilator Hydrolyzed collagen
-
 ?
3.4.21.32 N-acetyl-L-Ala-L-Ala-L-Ala-p-nitroanilide + H2O
-
 Leptuca pugilator ?
-
 ?
3.4.21.32 N-benzoyl-L-Arg-p-nitroanilide + H2O
-
 Leptuca pugilator ?
-
 ?
3.4.21.32 N-benzoyl-L-Tyr ethyl ester + H2O
-
 Leptuca pugilator ?
-
 ?
3.4.21.32 N-benzoyl-L-Tyr-p-nitroanilide + H2O
-
 Leptuca pugilator ?
-
 ?
3.4.21.32 N-benzoyl-L-Val-Gly-L-Arg-p-nitroanilide + H2O
-
 Leptuca pugilator ?
-
 ?
3.4.21.32 Nalpha-tosyl-L-Arg methyl ester + H2O
-
 Leptuca pugilator ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.32 monomer 1 * 25000, SDS-PAGE Leptuca pugilator