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Literature summary extracted from
- Purification and characterization of carboxypeptidases A and B from the white shrimp (Penaeus setiferus) (1973), Biochemistry, 12, 1867-1874.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.17.1 | no effect of freezing and thawing | Penaeus setiferus |
| 3.4.17.2 | at low protein concentration, 0.05 mg/ml, stable for at least 1 month | Penaeus setiferus |
| 3.4.17.2 | freezing and thawing has no effect | Penaeus setiferus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.1 | 1,10-phenanthroline | - |
Penaeus setiferus |  |
| 3.4.17.1 | 3-Phenylpropionic acid | - |
Penaeus setiferus | |
| 3.4.17.1 | Chelating agents | - |
Penaeus setiferus | |
| 3.4.17.2 | 1,10-phenanthroline | - |
Penaeus setiferus | |
| 3.4.17.2 | 3-Phenylpropanoic acid | - |
Penaeus setiferus | |
| 3.4.17.2 | 6-aminohexanoic acid | - |
Penaeus setiferus | |
| 3.4.17.2 | Beta-phenylpropionic acid | - |
Penaeus setiferus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.1 | 0.2 | - |
hippuryl-L-Arg | - |
Penaeus setiferus | |
| 3.4.17.1 | 0.33 | - |
hippuryl-L-Phe | - |
Penaeus setiferus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.1 | Zn2+ | metalloenzyme | Penaeus setiferus | |
| 3.4.17.2 | Zinc | contains zinc | Penaeus setiferus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 30000 | - |
boundary depletion sedimentation equilibrium | Penaeus setiferus |
| 3.4.17.2 | 31000 | - |
1 * 31000, SDS-PAGE | Penaeus setiferus |
| 3.4.17.2 | 34200 | - |
boundary depletion equilibrium sedimentation | Penaeus setiferus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.1 | Penaeus setiferus | - |
- |
- |
| 3.4.17.2 | Penaeus setiferus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.1 | - |
Penaeus setiferus |
| 3.4.17.2 | - |
Penaeus setiferus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | additional information | - |
- |
Penaeus setiferus |
| 3.4.17.2 | additional information | - |
- |
Penaeus setiferus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.1 | Hippuryl-L-Arg + H2O | - |
Penaeus setiferus | Hippuric acid + L-Arg | - |
ir | |
| 3.4.17.1 | hippuryl-L-Phe + H2O | - |
Penaeus setiferus | hippuric acid + L-Phe | - |
ir | |
| 3.4.17.2 | Arg-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | bradykinin | Penaeus setiferus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.1 | More | amino acid composition described | Penaeus setiferus |
| 3.4.17.2 | monomer | 1 * 31000, SDS-PAGE | Penaeus setiferus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 25 | - |
assay at | Penaeus setiferus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 6 | 8 | esterase activity, broad optimum | Penaeus setiferus |
| 3.4.17.1 | 7.5 | - |
assay at | Penaeus setiferus |
| 3.4.17.2 | 6.5 | 8 | - |
Penaeus setiferus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 6 | - |
stable at or above, unstable below | Penaeus setiferus |
| 3.4.17.2 | 6 | - |
or above, 6.0 mg/ml protein concentration, stable for several months | Penaeus setiferus |
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