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Literature summary extracted from

  • Wu, Z.; Walsh, C.T.
    Phosphinate analogs of D-, D-dipeptides: slow-binding inhibition and proteolysis protection of VanX, a D-, D-dipeptidase required for vancomycin resistance in Enterococcus faecium (1995), Proc. Natl. Acad. Sci. USA, 92, 11603-11607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.22 overexpression in Escherichia coli Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.22 Cu2+ 96% inhibition at 1 mM Enterococcus faecium
3.4.13.22 D-3-[(1-aminoethyl)phosphinyl]-D-2-methylpropionic acid phosphinate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction, slow binding Enterococcus faecium
3.4.13.22 D-Ala-L-Ala
-
 Enterococcus faecium
3.4.13.22 L-Ala-D-Ala
-
 Enterococcus faecium
3.4.13.22 L-Ala-L-Ala
-
 Enterococcus faecium
3.4.13.22 Zn2+ 72% inhibition at 1 mM Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.22 additional information
-
 additional information kinetics Enterococcus faecium
3.4.13.22 1.37
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.22 Co2+ 1.2fold activation at 1 mM Enterococcus faecium
3.4.13.22 Ni2+ 1.3fold activation at 1 mM Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O Enterococcus faecium enzyme is essential for vancomycin resistance in Enterococcus faecium D-Ala + D-Ala
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus faecium
-
-
-
3.4.13.22 Enterococcus faecium BM4147
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.22 recombinant from Escherichia coli, to homogeneity Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?
3.4.13.22 D-Ala-D-Ala + H2O enzyme is essential for vancomycin resistance in Enterococcus faecium Enterococcus faecium D-Ala + D-Ala
-
 ?
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecium BM4147 D-Ala + D-Ala
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 D-Ala-D-Ala-dipeptidase
-
 Enterococcus faecium
3.4.13.22 M15.011 Merops-ID Enterococcus faecium
3.4.13.22 VanX
-
 Enterococcus faecium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.22 37
-
 assay at Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.22 60
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.22 8
-
 assay at Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.13.22 additional information
-
 additional information inhibition kinetics, Ki is 0.0015 mM immediately after addition of the enzyme, but is then lowered to by a relatively slow isomerization step to a second complex to Ki = 0.00047 mM Enterococcus faecium
3.4.13.22 58
-
 D-Ala-L-Ala pH 8.0, 37°C Enterococcus faecium
3.4.13.22 98
-
 L-Ala-L-Ala pH 8.0, 37°C Enterococcus faecium
3.4.13.22 225
-
 L-Ala-D-Ala pH 8.0, 37°C Enterococcus faecium