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Literature summary extracted from
- Dipeptidyl peptidase I from goat brain: purification, characterization and its action on Leu-enkephalin (1993), Neurochem. Int., 22, 59-68.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.1 | dithioerythritol | 5 mM, activates | Capra hircus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.1 | antipain | - |
Capra hircus | |
| 3.4.14.1 | iodoacetamide | - |
Capra hircus | |
| 3.4.14.1 | iodoacetate | - |
Capra hircus | |
| 3.4.14.1 | L-trans-epoxy-succinyl-leucylamido(4-guanidino)-butane | - |
Capra hircus | |
| 3.4.14.1 | leupeptin | - |
Capra hircus | |
| 3.4.14.1 | NEM | - |
Capra hircus | |
| 3.4.14.1 | PCMB | - |
Capra hircus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.1 | 0.1 | - |
Gly-Arg-2-naphthylamide | - |
Capra hircus | |
| 3.4.14.1 | 0.14 | - |
Gly-Arg-4-methoxy-beta-naphthylamide | - |
Capra hircus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.1 | Cl- | 20 mM, required for maximal activity | Capra hircus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 22000 | - |
x * 22000, SDS-PAGE in presence of 2-mercaptoethanol | Capra hircus |
| 3.4.14.1 | 180000 | - |
gel filtration | Capra hircus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.1 | Capra hircus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.1 | - |
Capra hircus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.14.1 | brain | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 3.019 | - |
- |
Capra hircus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.1 | Gly-Arg-2-naphthylamide + H2O | - |
Capra hircus | Gly-Arg + 2-naphthylamine | - |
? | |
| 3.4.14.1 | Gly-Arg-4-methoxy-2-naphthylamide + H2O | - |
Capra hircus | Gly-Arg + 4-methoxy-2-naphthylamine | - |
? | |
| 3.4.14.1 | Leu-enkephalin + H2O | i.e. Tyr-Gly-Gly-Phe-Leu | Capra hircus | Tyr-Gly + Gly-Phe-Leu | no further degradation of the tripeptide | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.1 | ? | x * 22000, SDS-PAGE in presence of 2-mercaptoethanol | Capra hircus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 50 | - |
- |
Capra hircus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 50 | - |
at pH 4.6, stable up to | Capra hircus |
| 3.4.14.1 | 60 | - |
at pH 5.5, stable up to | Capra hircus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 6 | - |
Gly-Arg-4-methoxy-beta-naphthylamide as substrate | Capra hircus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.1 | 4.5 | 8 | stable | Capra hircus |
| 3.4.14.1 | 4.6 | - |
stable up to 50°C | Capra hircus |
| 3.4.14.1 | 5.5 | - |
stable up to 60°C | Capra hircus |
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