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Literature summary extracted from
- Characterization of a prolidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 with an unusual regulation of biosynthesis (1999), Microbiology, 145, 437-446.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.13.9 | expression in Escherichia coli | Lactobacillus delbrueckii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | bestatin | - |
Lactobacillus delbrueckii |  |
| 3.4.13.9 | dithiothreitol | - |
Lactobacillus delbrueckii | |
| 3.4.13.9 | EDTA | - |
Lactobacillus delbrueckii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.13.9 | 2.2 | - |
Leu-Pro | - |
Lactobacillus delbrueckii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.9 | Zn2+ | requirement | Lactobacillus delbrueckii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 50000 | - |
2 * 50000, SDS-PAGE | Lactobacillus delbrueckii |
| 3.4.13.9 | 68000 | 70000 | gel filtration | Lactobacillus delbrueckii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.13.9 | Lactobacillus delbrueckii | - |
subsp. bulgaricus CNRZ 397 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.13.9 | - |
Lactobacillus delbrueckii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 446 | - |
- |
Lactobacillus delbrueckii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.13.9 | Arg-Pro + H2O | - |
Lactobacillus delbrueckii | Arg + Pro | - |
? | |
| 3.4.13.9 | L-Ala-Pro + H2O | - |
Lactobacillus delbrueckii | L-Ala + Pro | - |
? | |
| 3.4.13.9 | L-Phe-Pro + H2O | - |
Lactobacillus delbrueckii | L-Phe + Pro | - |
? | |
| 3.4.13.9 | L-Val-Pro + H2O | - |
Lactobacillus delbrueckii | L-Val + Pro | - |
? | |
| 3.4.13.9 | Leu-Pro + H2O | - |
Lactobacillus delbrueckii | Leu + Pro | - |
? | |
| 3.4.13.9 | additional information | no substrate: Pro-Pro, Gly-Pro | Lactobacillus delbrueckii | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.13.9 | dimer | 2 * 50000, SDS-PAGE | Lactobacillus delbrueckii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 50 | - |
- |
Lactobacillus delbrueckii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.13.9 | 6 | - |
- |
Lactobacillus delbrueckii |
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Release 2023.1 (January 2023)
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