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Literature summary extracted from

  • Simmons, W.H.; Orawski, A.T.
    Membrane-bound aminopeptidase P from bovine lung. Its purification, properties, and degradation of bradykinin (1992), J. Biol. Chem., 267, 4897-4903.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 2-mercaptoethanol
-
 Bos taurus
3.4.11.9 NaCl above 0.25 M Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.9 0.076
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Bos taurus
3.4.11.9 0.15
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Bos taurus
3.4.11.9 0.15
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Bos taurus
3.4.11.9 0.25
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Bos taurus
3.4.11.9 0.34
-
 Arg-Pro-Pro-Gly-Phe
-
 Bos taurus
3.4.11.9 0.76
-
 Arg-Pro-Pro
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.9 membrane attached to the membranes via a glycosylphosphatidylinositol anchor Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 95000
-
 x * 95000, reducing conditions, SDS-PAGE Bos taurus
3.4.11.9 360000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.9 additional information Bos taurus the enzyme is involved in the pulmonary inactivation of circulating bradykinin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.9 side-chain modification the enzyme is attached to the membranes via a glycosylphosphatidylinositol anchor Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.9 lung
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.4.11.9 -20°C, pH 6.8, stable for at least 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 Arg-Pro-Pro + H2O
-
 Bos taurus Arg + Pro-Pro
-
 ?
3.4.11.9 Arg-Pro-Pro-Gly-Phe + H2O
-
 Bos taurus ?
-
 ?
3.4.11.9 Arg-Pro-Pro-Gly-Phe-Ser + H2O
-
 Bos taurus ?
-
 ?
3.4.11.9 Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O
-
 Bos taurus ?
-
 ?
3.4.11.9 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + H2O
-
 Bos taurus ?
-
 ?
3.4.11.9 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin Bos taurus Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.11.9 additional information the enzyme favors peptides with 2 proline residues or proline analogs in position 2 and 3 of the substrate Bos taurus ?
-
 ?
3.4.11.9 additional information the enzyme is involved in the pulmonary inactivation of circulating bradykinin Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 tetramer x * 95000, reducing conditions, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.9 60
-
 pH 6.8, 30 min, stable up to Bos taurus
3.4.11.9 75
-
 pH 6.8, 30 min, complete loss of activity Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.9 26
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Bos taurus
3.4.11.9 30
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Bos taurus
3.4.11.9 39
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Bos taurus
3.4.11.9 41
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Bos taurus
3.4.11.9 48
-
 Arg-Pro-Pro-Gly-Phe
-
 Bos taurus
3.4.11.9 120
-
 Arg-Pro-Pro
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 6.5
-
 hydrolysis of Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg Bos taurus
3.4.11.9 7.5
-
-
 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.9 8 11 37°C, 2 h, maximal stability Bos taurus