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Literature summary extracted from

  • Yoshimoto, T.; Murayama, N.; Honda, T.; Tone, H.; Tsuru, D.
    Cloning and expression of aminopeptidase P gene from Escherichia coli HB101 and characterization of expressed enzyme (1988), J. Biochem., 104, 93-97.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.9
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.9 antipain
-
 Escherichia coli
3.4.11.9 CH3HgCl
-
 Escherichia coli
3.4.11.9 EDTA
-
 Escherichia coli
3.4.11.9 PCMB partial Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.9 Mn2+ 0.005 mM, 3fold activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.9 50000
-
 4 * 50000, SDS-PAGE Escherichia coli
3.4.11.9 200000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.9 Escherichia coli
-
 HB101
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.9
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.9 3.45
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.9 Ala-Pro-p-nitroanilide + H2O
-
 Escherichia coli Ala + Pro-p-nitroanilide
-
 ?
3.4.11.9 Glu-Pro-p-nitroanilide + H2O
-
 Escherichia coli Glu + Pro-p-nitroanilide
-
 ?
3.4.11.9 Gly-Pro-2-naphthylamide + H2O
-
 Escherichia coli Gly + Pro-2-naphthylamide
-
 ?
3.4.11.9 His-Pro-p-nitroanilide + H2O
-
 Escherichia coli His + Pro-p-nitroanilide
-
 ?
3.4.11.9 Leu-Pro + H2O
-
 Escherichia coli Leu + Pro
-
 ?
3.4.11.9 Lys-Pro-p-nitroanilide + H2O
-
 Escherichia coli Lys + Pro-p-nitroanilide
-
 ?
3.4.11.9 Met-Pro + H2O
-
 Escherichia coli Met + Pro
-
 ?
3.4.11.9 Phe-Pro + H2O
-
 Escherichia coli Phe + Pro
-
 ?
3.4.11.9 Pro-Pro-Gly-(Pro-Pro-Gly)4 + H2O
-
 Escherichia coli ?
-
 ?
3.4.11.9 Ser-Pro-p-nitroanilide + H2O
-
 Escherichia coli Ser + Pro-p-nitroanilide
-
 ?
3.4.11.9 Val-Pro + H2O
-
 Escherichia coli Val + Pro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.9 tetramer 4 * 50000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.9 50
-
 pH 8.0, 30 min, 50% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.9 8.5
-
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.9 8
-
 50°C, 30 min, 50% loss of activity Escherichia coli
3.4.11.9 8 9 stable Escherichia coli