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Literature summary extracted from

  • Menguito, C.A.; Papaconstantinou, J.; Weigel, P.H.
    The histidyl-tRNA synthetase from Streptococcus equisimilis: overexpression in Escherichia coli. Purification and characterization (1993), Prep. Biochem., 23, 499-472.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 overexpression in Escherichia coli of a fusion HisRS, with additional 15 amino acids between the initiator Met and the second amino acid Lys Streptococcus dysgalactiae subsp. equisimilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 53700
-
 2 * 53700, SDS-PAGE with or without reduction Streptococcus dysgalactiae subsp. equisimilis
6.1.1.21 94000
-
 gel filtration, active fusion HisRS Streptococcus dysgalactiae subsp. equisimilis
6.1.1.21 108200
-
 nondenaturing PAGE, active fusion HisRS Streptococcus dysgalactiae subsp. equisimilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Streptococcus dysgalactiae subsp. equisimilis
-
 native HisRS and fusion HisRS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21 native HisRS and fusion HisRS Streptococcus dysgalactiae subsp. equisimilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Streptococcus dysgalactiae subsp. equisimilis AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 dimer 2 * 53700, SDS-PAGE with or without reduction Streptococcus dysgalactiae subsp. equisimilis