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Literature summary extracted from

  • Leiter, H.; Mucha, J.; Staudacher, E.; Grimm, R.; Glossl, J.; Altmann, F.
    Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans (1999), J. Biol. Chem., 274, 21830-21839.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.214 cDNA cloning of Fuc-T C3 from mRNA, recombinant enzyme expressed in Sf21 insect cells using baculovirus vector Vigna radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.214 0.11
-
 GDP-fucose
-
 Vigna radiata
2.4.1.214 0.13
-
 GnGnF6-peptide
-
 Vigna radiata
2.4.1.214 0.19
-
 GnGn-peptide
-
 Vigna radiata
2.4.1.214 0.23
-
 M5Gn-Asn
-
 Vigna radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.214 microsome
-
 Vigna radiata
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 54000
-
 x * 54000, major isoform, SDS-PAGE Vigna radiata
2.4.1.214 56000
-
 x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE Vigna radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.214 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Vigna radiata
-
 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 of Fuc-T C3, using Triton X-100 extraction and column chromatography on DE52 cellulose, Affi-Gel blue, S-Sepharose, GnGn-Sepharose and GDP-hexanolamine-Sepharose Vigna radiata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.214 43.35
-
-
 Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.214 GDP-fucose + dabsylated-GalGal-peptide 0.7% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
 ?
2.4.1.214 GDP-fucose + dabsylated-GalGn-peptide 50% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
 ?
2.4.1.214 GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn
-
 Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcbeta1-Asn
-
 ?
2.4.1.214 GDP-fucose + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn
-
 Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn
-
 ?
2.4.1.214 GDP-fucose + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4GlcNAcbeta1-Asn
-
 Vigna radiata GDP + GlcNAcbeta1-2-Manalpha1-3[Manalpha1-3(Manalpha1-6)Manalpha1-6]Manbeta1-4-GlcNAcbeta1-4 (Fucalpha1-3)GlcNAcbeta1-Asn
-
 ?
2.4.1.214 GDP-fucose + GnGn-peptide 100% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated peptide
-
 ?
2.4.1.214 GDP-fucose + GnGnF6-peptide 87% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated GnGnF6-peptide
-
 ?
2.4.1.214 GDP-fucose + M5Gn-Asn 71% relative activity with recombinant enzyme Vigna radiata GDP + alpha1-3-fucosylated M5Gn-Asn
-
 ?
2.4.1.214 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Vigna radiata GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.214 ? x * 54000, major isoform, SDS-PAGE Vigna radiata
2.4.1.214 ? x * 56000, represents the same enzyme as the band with 54kDA, SDS-PAGE Vigna radiata