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Literature summary extracted from
- Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethylene (PCE) from Clostridium bifermentans DPH-1 (2001), Can. J. Microbiol., 47, 448-456.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | agriculture | can play important role in breakdown of perchloroethene and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances | Paraclostridium bifermentans |
| 1.21.99.5 | agriculture | bioremediation of chloroethylenes, multiple chlorinated aliphatic molecules, in contaminated sites and water, anaerobic reductive dehalogenation of toxic xenobiotic | Paraclostridium bifermentans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | - |
Paraclostridium bifermentans |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.21.99.5 | enzyme generally unstable | Paraclostridium bifermentans |
| 1.21.99.5 | enzyme oxygen sensitive | Paraclostridium bifermentans |
| 1.21.99.5 | purified enzyme not stable, 50% loss of activity at -30°C | Paraclostridium bifermentans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | EDTA | no effect, no inhibition | Paraclostridium bifermentans |  |
| 1.21.99.5 | Propyl iodide | 1-iodopropane; 90% inhibition by mixture of propyl iodide and titanium citrate in the dark, light restores about 95% of initial activity; light-reversible inactivation in the presence of titanium(III)citrate as electron donor | Paraclostridium bifermentans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.99.5 | additional information | - |
additional information | enzyme kinetics and kinetic parameters | Paraclostridium bifermentans | |
| 1.21.99.5 | 0.012 | - |
tetrachloroethene | - |
Paraclostridium bifermentans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.21.99.5 | cytoplasmic membrane | not an integral membrane protein but a peripheral membrane protein | Paraclostridium bifermentans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Cobalamin | cobalamin-containing enzyme | Paraclostridium bifermentans | |
| 1.21.99.5 | Cobalamin | no significant effect of cyanocobalamin | Paraclostridium bifermentans | |
| 1.21.99.5 | Cobalt | Co2+ corrinoid | Paraclostridium bifermentans | |
| 1.21.99.5 | additional information | independent of metal ions | Paraclostridium bifermentans |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 35000 | - |
2 * 35000, homodimer, SDS-PAGE | Paraclostridium bifermentans |
| 1.21.99.5 | 35700 | - |
2 * 35700, homodimer, MALDI-TOF/MS | Paraclostridium bifermentans |
| 1.21.99.5 | 70000 | - |
gel filtration | Paraclostridium bifermentans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + reduced acceptor | Paraclostridium bifermentans | - |
trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | Paraclostridium bifermentans DPH-1 | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.99.5 | Paraclostridium bifermentans | - |
DPH-1 | - |
| 1.21.99.5 | Paraclostridium bifermentans DPH-1 | - |
DPH-1 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | proteolytic modification | - |
Paraclostridium bifermentans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | - |
Paraclostridium bifermentans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 0.086 | - |
- |
Paraclostridium bifermentans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.21.99.5 | -30°C, purified enzyme not stable, 50% loss of activity | Paraclostridium bifermentans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | 1,1,2-trichloroethane + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1.21.99.5 | 1,1-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1.21.99.5 | 1,2-dichloropropane + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1.21.99.5 | cis-1,2-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1.21.99.5 | additional information | substrate specificity | Paraclostridium bifermentans | ? | - |
? | |
| 1.21.99.5 | additional information | broad substrate specificity, degrades various chlorinated aliphatic compounds | Paraclostridium bifermentans | ? | - |
? | |
| 1.21.99.5 | additional information | substrate specificity | Paraclostridium bifermentans DPH-1 | ? | - |
? | |
| 1.21.99.5 | additional information | broad substrate specificity, degrades various chlorinated aliphatic compounds | Paraclostridium bifermentans DPH-1 | ? | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | - |
Paraclostridium bifermentans | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Paraclostridium bifermentans | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | - |
Paraclostridium bifermentans DPH-1 | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Paraclostridium bifermentans DPH-1 | trichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | trans-1,2-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1.21.99.5 | trichloroethene + reduced acceptor | - |
Paraclostridium bifermentans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
| 1.21.99.5 | trichloroethene + reduced acceptor | - |
Paraclostridium bifermentans DPH-1 | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | dimer | 2 * 35000, homodimer, SDS-PAGE | Paraclostridium bifermentans |
| 1.21.99.5 | dimer | 2 * 35700, homodimer, MALDI-TOF/MS | Paraclostridium bifermentans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 30 | - |
assay at | Paraclostridium bifermentans |
| 1.21.99.5 | 35 | - |
- |
Paraclostridium bifermentans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 20 | 35 | relatively stable between | Paraclostridium bifermentans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 7.5 | - |
- |
Paraclostridium bifermentans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 7.5 | 8 | most stable between | Paraclostridium bifermentans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | corrinoid | Co2+ corrinoid | Paraclostridium bifermentans | |
| 1.21.99.5 | additional information | no effect by NADH | Paraclostridium bifermentans |
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