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Literature summary extracted from

  • Neumann, A.; Wohlfarth, G.; Diekert, G.
    Properties of tetrachloroethene and trichloroethene dehalogenase of Dehalospirillum multivorans (1995), Arch. Microbiol., 163, 276-281.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.21.99.5 agriculture uesd to decontaminate environment polluntant, tetrachloroethene, by tetrachloroethene bioremediation Sulfurospirillum multivorans

General Stability

EC Number General Stability Organism
1.21.99.5 enzyme insensitive towards oxygen Sulfurospirillum multivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.5 cyanide 0.1 mM CN-: about 50% inhibition Sulfurospirillum multivorans
1.21.99.5 EDTA 1 mM: 50% inhibition Sulfurospirillum multivorans
1.21.99.5 KCN
-
 Sulfurospirillum multivorans
1.21.99.5 additional information no inhibition by carbon monoxide, azide, hydroxylamine, nitrate, cis-1,2-dichloroethene, trans-1,2-dichloroethene or 1,1-dichloroethene Sulfurospirillum multivorans
1.21.99.5 N2O dinitrogen oxide; N2O, 100%, present in the gas phase: complete inhibition Sulfurospirillum multivorans
1.21.99.5 nitrite strong inhibition, 1 mM NO2-: complete inhibition Sulfurospirillum multivorans
1.21.99.5 Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation; light-reversible inactivation in the presence of titanium(III)citrate as electron donor Sulfurospirillum multivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.5 additional information
-
 additional information enzyme kinetics and kinetic parameters Sulfurospirillum multivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.99.5 Cobalt Co(III) Sulfurospirillum multivorans
1.21.99.5 additional information indicates the presence of a second transition metal Sulfurospirillum multivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.99.5 tetrachloroethene + reduced acceptor Sulfurospirillum multivorans
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Sulfurospirillum multivorans
-
 strictly anaerobic, gram-negative bacterium
-
1.21.99.5 Sulfurospirillum multivorans
-
 tetrachloroethene-utilizing anaerobic bacterium
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.99.5 trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor mechanism Sulfurospirillum multivorans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 tetrachloroethene + reduced acceptor
-
 Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 trichloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Sulfurospirillum multivorans cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.99.5 25
-
 assay at Sulfurospirillum multivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.99.5 7.5
-
 assay at Sulfurospirillum multivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.99.5 corrinoid vitamin B12 Sulfurospirillum multivorans
1.21.99.5 vitamin B12
-
 Sulfurospirillum multivorans