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Literature summary extracted from

  • Oka, M.; Yang, Y.S.; Nagata, S.; Esaki, N.; Tanaka, H.; Soda, K.
    Overproduction of thermostable leucine dehydrogenase of Bacillus stearothermophilus and its one-step purification from recombinant cells of Escherichia coli (1989), Biotechnol. Appl. Biochem., 11, 307-311.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 expression in Escherichia coli Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9 one-step purification of recombinant enzyme Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 120
-
-
 Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
1.4.1.9 4°C, buffer containing 0.02% sodium azide, stable for more than 1 year Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 L-Leu + H2O + NAD+
-
 Geobacillus stearothermophilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 55
-
 pH 6.0-11.0, 10 min, stable Geobacillus stearothermophilus
1.4.1.9 70
-
 30 min, retains full activity Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 6 11 55°C, 10 min, stable Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
 Geobacillus stearothermophilus
1.4.1.9 NADH
-
 Geobacillus stearothermophilus