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Literature summary extracted from

  • Livesey, G.; Lund, P.
    Isolation and characterization of leucine dehydrogenase from Bacillus subtilis (1988), Methods Enzymol., 166, 282-288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.9 L-Leu competitive inhibition of the reductive amination of 4-methylthio-2-oxobutanoate Bacillus subtilis
1.4.1.9 Mn2+ inhibits oxidative deamination Bacillus subtilis
1.4.1.9 NH4+ inhibits oxidative deamination Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.6
-
4-methyl-2-oxopentanoate
-
Bacillus subtilis
1.4.1.9 0.9
-
2-oxo-3-methylpentanoate
-
Bacillus subtilis
1.4.1.9 1.33
-
L-Leu
-
Bacillus subtilis
1.4.1.9 1.5
-
NAD+
-
Bacillus subtilis
1.4.1.9 2.4
-
L-Ile
-
Bacillus subtilis
1.4.1.9 3.3
-
3-methyl-2-oxobutanoate
-
Bacillus subtilis
1.4.1.9 3.4
-
L-Val
-
Bacillus subtilis
1.4.1.9 6.7
-
4-(methylsulfanyl)-2-oxobutanoate
-
Bacillus subtilis
1.4.1.9 25
-
L-Met
-
Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 15.23
-
-
Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
-
Bacillus subtilis L-methionine + H2O + NAD+
-
r
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
?
1.4.1.9 L-Ile + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Leu + H2O + NAD+
-
Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
1.4.1.9 L-Met + H2O + NAD+
-
Bacillus subtilis 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
r
1.4.1.9 L-norleucine + H2O + NAD+
-
Bacillus subtilis 2-oxohexanoate + NH3 + NADH
-
?
1.4.1.9 L-Val + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9.5
-
reductive amination of 4-methyl-2-oxobutanoate Bacillus subtilis
1.4.1.9 11
-
oxidative deamination of L-Leu Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
Bacillus subtilis
1.4.1.9 NADH
-
Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.9 1
-
L-Leu competitive inhibition of the reductive amination of 4-methylthio-2-oxobutanoate Bacillus subtilis