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Literature summary extracted from

  • Laue, H.; Cook, A.M.
    Purification, properties and primary structure of alanine dehydrogenase involved in taurine metabolism in the anaerobe Bilophila wadsworthia (2000), Arch. Microbiol., 174, 162-167.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.1 repeated freezing and thawing leads to significant loss of activity Bilophila wadsworthia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.04
-
 NADH
-
 Bilophila wadsworthia
1.4.1.1 0.15
-
 NAD+
-
 Bilophila wadsworthia
1.4.1.1 1.1
-
 pyruvate
-
 Bilophila wadsworthia
1.4.1.1 1.6
-
 L-Ala
-
 Bilophila wadsworthia
1.4.1.1 31
-
 NH4+
-
 Bilophila wadsworthia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 39900
-
 6 * 39900, calculation from nucleotide sequence Bilophila wadsworthia
1.4.1.1 42000
-
 6 * 42000, SDS-PAGE Bilophila wadsworthia
1.4.1.1 273000
-
 gel filtration Bilophila wadsworthia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 additional information Bilophila wadsworthia enzyme is involved in taurine metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bilophila wadsworthia Q9AIK2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Bilophila wadsworthia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 additional information
-
-
 Bilophila wadsworthia

Storage Stability

EC Number Storage Stability Organism
1.4.1.1 -20°C, stable for at least 1 month Bilophila wadsworthia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 D-Ala + H2O + NAD+ 3.5% of the activity with L-Ala Bilophila wadsworthia pyruvate + NH3 + NADH
-
 ?
1.4.1.1 D-Ser + H2O + NAD+ 4.4% of the activity with L-Ala Bilophila wadsworthia 3-hydroxypyruvate + NH3 + NADH
-
 r
1.4.1.1 D-Ser + H2O + NAD+ in the reverse reaction the enzyme reacts at 9.3% of the activity with pyruvate Bilophila wadsworthia 3-hydroxypyruvate + NH3 + NADH
-
 r
1.4.1.1 glyoxylate + NH3 + NADH
-
 Bilophila wadsworthia aminoacetate + NAD+ + H2O
-
 ?
1.4.1.1 L-2-aminobutanoate + H2O + NAD+ in the reverse reaction the enzyme reacts at 15.3% of the activity with pyruvate Bilophila wadsworthia 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.1 L-2-aminobutanoate + H2O + NAD+ 10.4% of the activity with L-Ala Bilophila wadsworthia 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Bilophila wadsworthia pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ile + H2O + NAD+
-
 Bilophila wadsworthia 2-oxo-3-methylpentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-norvaline + H2O + NAD+ at 4.6% of the activity with L-Ala Bilophila wadsworthia 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.1 L-Val + H2O + NAD+ 4.8% of the activity with L-Ala Bilophila wadsworthia 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.1 additional information no activity with taurine Bilophila wadsworthia ?
-
 ?
1.4.1.1 additional information enzyme is involved in taurine metabolism Bilophila wadsworthia ?
-
 ?
1.4.1.1 oxaloacetate + NH3 + NADH
-
 Bilophila wadsworthia L-Asp + H2O + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer 6 * 42000, SDS-PAGE Bilophila wadsworthia
1.4.1.1 hexamer 6 * 39900, calculation from nucleotide sequence Bilophila wadsworthia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 50 55 oxidative deamination Bilophila wadsworthia
1.4.1.1 55 60 reductive amination Bilophila wadsworthia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 60
-
 inactivation after about 1 min Bilophila wadsworthia
1.4.1.1 65
-
 inactivation after about 10 s Bilophila wadsworthia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9
-
 reductive amination of pyruvate Bilophila wadsworthia
1.4.1.1 9 11.5 oxidative deamination of L-Ala Bilophila wadsworthia

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Bilophila wadsworthia
1.4.1.1 NADH
-
 Bilophila wadsworthia