Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ohashima, T.; Soda, K.
    Purification and properties of alanine dehydrogenase from Bacillus sphaericus (1979), Eur. J. Biochem., 100, 29-39.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.1
-
Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Cu2+
-
Lysinibacillus sphaericus
1.4.1.1 HgCl2
-
Lysinibacillus sphaericus
1.4.1.1 Pb2+
-
Lysinibacillus sphaericus
1.4.1.1 PCMB
-
Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.014
-
L-norvaline
-
Lysinibacillus sphaericus
1.4.1.1 0.018
-
L-Ala
-
Lysinibacillus sphaericus
1.4.1.1 0.02
-
L-Val
-
Lysinibacillus sphaericus
1.4.1.1 0.039
-
L-Ser
-
Lysinibacillus sphaericus
1.4.1.1 0.33
-
L-2-aminobutanoate
-
Lysinibacillus sphaericus
1.4.1.1 1.7
-
pyruvate
-
Lysinibacillus sphaericus
1.4.1.1 11
-
2-oxobutanoate
-
Lysinibacillus sphaericus
1.4.1.1 11
-
2-oxo-3-methylbutanoate
-
Lysinibacillus sphaericus
1.4.1.1 12
-
glyoxylate
-
Lysinibacillus sphaericus
1.4.1.1 23
-
2-oxopentanoate
-
Lysinibacillus sphaericus
1.4.1.1 80
-
3-hydroxypyruvate
-
Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 230000
-
gel filtration Lysinibacillus sphaericus
1.4.1.1 231000
-
equilibrium sedimentation Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
Lysinibacillus sphaericus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.1 L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+ sequential ordered ternary-binary mechanism, the enzyme is A-stereospecific, the pro-R hydrogen at C-4 of the reduced nicotinamide ring of NADH is exclusively transferred to pyruvate Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 157
-
-
Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
1.4.1.1 4°C, 80% saturated ammonium sulfate suspension, pH 7-8, 2 years, stable Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-oxo-3-methylbutanoate + NH3 + NADH
-
Lysinibacillus sphaericus L-Val + NAD+ + H2O
-
?
1.4.1.1 2-oxobutanoate + NH3 + NADH
-
Lysinibacillus sphaericus 2-aminobutanoate + H2O + NAD+
-
r
1.4.1.1 glyoxylate + NH3 + NADH
-
Lysinibacillus sphaericus aminoacetate + NAD+ + H2O
-
?
1.4.1.1 L-2-aminobutanoate + H2O + NAD+
-
Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.1 L-Ala + H2O + NAD+
-
Lysinibacillus sphaericus pyruvate + NH3 + NADH
-
r
1.4.1.1 L-norvaline + H2O + NAD+
-
Lysinibacillus sphaericus 2-oxopentanoate + NH3 + NADH
-
r
1.4.1.1 L-Ser + H2O + NAD+
-
Lysinibacillus sphaericus 3-hydroxypyruvate + NH3 + NADH
-
r
1.4.1.1 L-Val + H2O + NAD+
-
Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 70
-
increase of activity up to Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 50
-
pH 6.0-10.0, 5 min, stable Lysinibacillus sphaericus
1.4.1.1 60
-
pH 7.2, 10 mM potassium phosphate buffer, 5 min, stable up to Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9
-
reductive amination Lysinibacillus sphaericus
1.4.1.1 10 10.5 oxidative deamination Lysinibacillus sphaericus
1.4.1.1 10.5
-
deamination of L-Ala Lysinibacillus sphaericus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.1 6 10 50°C, 5 min, stable Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
Lysinibacillus sphaericus
1.4.1.1 NADH
-
Lysinibacillus sphaericus