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Literature summary extracted from

  • Aharonowitz, Y.; Friedrich, C.G.
    Alanine dehydrogenase of the beta-lactam antibiotic producer Streptomyces clavuligerus (1980), Arch. Microbiol., 125, 137-142.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.1 93% loss of activity after dialysis Streptomyces clavuligerus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 2-oxobutanoate weak Streptomyces clavuligerus
1.4.1.1 3-hydroxypyruvate competitive with respect to pyruvate Streptomyces clavuligerus
1.4.1.1 Cu2+
-
 Streptomyces clavuligerus
1.4.1.1 D-Ala aminating reaction, competitive with respect to NADH, noncompetitive with respect to NH4+ and pyruvate Streptomyces clavuligerus
1.4.1.1 Fe2+
-
 Streptomyces clavuligerus
1.4.1.1 glyoxylate weak Streptomyces clavuligerus
1.4.1.1 Hg2+
-
 Streptomyces clavuligerus
1.4.1.1 Hydroxypyruvate
-
 Streptomyces clavuligerus
1.4.1.1 L-Ala reductive amination of pyruvate Streptomyces clavuligerus
1.4.1.1 L-Ser inhibition of aminating activity Streptomyces clavuligerus
1.4.1.1 NADPH inhibition of reductive amination Streptomyces clavuligerus
1.4.1.1 NH4+ inhibition of aminating activity Streptomyces clavuligerus
1.4.1.1 Zn2+
-
 Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.14
-
 NADH
-
 Streptomyces clavuligerus
1.4.1.1 0.5
-
 pyruvate
-
 Streptomyces clavuligerus
1.4.1.1 1.1
-
 pyruvate
-
 Streptomyces clavuligerus
1.4.1.1 9.1
-
 L-Ala
-
 Streptomyces clavuligerus
1.4.1.1 20
-
 NH4+
-
 Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 92000
-
 sucrose density gradient centrifugation Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Streptomyces clavuligerus

Storage Stability

EC Number Storage Stability Organism
1.4.1.1 4°C, 75% saturated ammonium sulfate suspension, purified enzyme stable for at least 12 months Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 2-oxobutanoate + NH3 + NADH no activity Streptomyces clavuligerus 2-aminobutanoate + H2O + NAD+
-
 ?
1.4.1.1 2-oxobutanoate + NH3 + NADH 3.7% of the activity with pyruvate Streptomyces clavuligerus 2-aminobutanoate + H2O + NAD+
-
 ?
1.4.1.1 3-hydroxypyruvate + NH3 + NADH 6.9% of the activity with pyruvate Streptomyces clavuligerus L-Ser + H2O + NAD+
-
 ?
1.4.1.1 glyoxylate + NH3 + NADH 5.6% of the activity with pyruvate Streptomyces clavuligerus aminoacetate + NAD+ + H2O
-
 ?
1.4.1.1 L-Ala + H2O + NAD+
-
 Streptomyces clavuligerus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ser + H2O + NAD+ no activity Streptomyces clavuligerus 3-hydroxypyruvate + NH3 + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8.4 8.6 pyruvate amination Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Streptomyces clavuligerus
1.4.1.1 NADH
-
 Streptomyces clavuligerus
1.4.1.1 NADP+ 8% of the activity with NAD+ Streptomyces clavuligerus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.1 1.6
-
 L-Ala with respect to NADH Streptomyces clavuligerus
1.4.1.1 2
-
 L-Ala with respect to NH4+ Streptomyces clavuligerus
1.4.1.1 3
-
 L-Ala with respect to pyruvate Streptomyces clavuligerus