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Literature summary extracted from

  • Muresan, L.; Vancea, D.; Presecan, E.; Porumb, H.; Lascu, I.; Oarga, M.; Matinca, D.; Abrudan, I.; Barzu, O.
    Catalytic properties of Sepharose-bound L-alanine dehydrogenase from Bacillus cereus (1983), Biochim. Biophys. Acta, 742, 617-622.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.1 urea, above 3 M, denaturation of soluble enzyme, resistance to denaturation increases by immobilization, 5 M, 30 min, 85% loss of activity of matrix-bound enzyme Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.037
-
 NADH soluble enzyme Bacillus cereus
1.4.1.1 0.18
-
 NAD+ soluble enzyme Bacillus cereus
1.4.1.1 0.32
-
 NADH Sepharose-bound enzyme Bacillus cereus
1.4.1.1 0.48
-
 pyruvate soluble enzyme Bacillus cereus
1.4.1.1 0.52
-
 pyruvate Sepharose-bound enzyme Bacillus cereus
1.4.1.1 1.18
-
 NAD+ Sepharose-bound enzyme Bacillus cereus
1.4.1.1 11.3
-
 L-Ala Sepharose-bound enzyme Bacillus cereus
1.4.1.1 12.5
-
 L-Ala soluble enzyme Bacillus cereus
1.4.1.1 22
-
 NH4+ Sepharose-bound enzyme Bacillus cereus
1.4.1.1 30
-
 NH4+ soluble enzyme Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 partial Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Bacillus cereus pyruvate + NH3 + NADH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 37
-
 28 d, immobilized enzyme retains 85% of its activity in 0.1 M phosphate buffer, pH 8 Bacillus cereus
1.4.1.1 59
-
 10 min, 50% loss of activity of soluble enzyme Bacillus cereus
1.4.1.1 65
-
 10 min, complete loss of activity, soluble enzyme Bacillus cereus
1.4.1.1 67
-
 10 min, 50% loss of activity, immobilized enzyme Bacillus cereus
1.4.1.1 75
-
 10 min, complete loss of activity, immobilized enzyme Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9 9.5 amination reaction, soluble and Sepharose-bound enzyme Bacillus cereus
1.4.1.1 10.5 11
-
 Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.1 6 9 25°C, 24 h, concentration 0.001 mg/ml, stable Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Bacillus cereus
1.4.1.1 NADH
-
 Bacillus cereus