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Literature summary extracted from

  • Shiotani, T.; Weber, G.
    Purification and properties of dihydrothymine dehydrogenase from rat liver (1981), J. Biol. Chem., 256, 219-224.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.2 2-mercaptoethanol stabilizes during dialysis and heating Rattus norvegicus
1.3.1.2 24 h dialysis against 35 mM potassium phosphate buffer, pH 7.4, with addition of 5 mM 2-mercaptoethanol or dithiothreitol, stable Rattus norvegicus
1.3.1.2 irreversible denaturation of enzyme in dilute solutions below 1 mg/ml Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.0018
-
 Uracil
-
 Rattus norvegicus
1.3.1.2 0.0026
-
 thymine
-
 Rattus norvegicus
1.3.1.2 0.0029
-
 NADP+ in presence of dihydrouracil Rattus norvegicus
1.3.1.2 0.0038
-
 NADP+ in presence of dihydrothymine Rattus norvegicus
1.3.1.2 0.011
-
 NADPH in presence of uracil Rattus norvegicus
1.3.1.2 0.015
-
 NADPH in presence of thymine Rattus norvegicus
1.3.1.2 0.043
-
 Dihydrothymine
-
 Rattus norvegicus
1.3.1.2 0.193
-
 dihydrouracil
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.2 Iron 1 mol of enzyme contains 3 mol of iron Rattus norvegicus
1.3.1.2 Iron iron atoms are probably present in iron-sulfur centers Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 110000
-
 2 * 110000, SDS-PAGE Rattus norvegicus
1.3.1.2 220000
-
 gel filtration, sedimentation equilibrium Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 thymine + NADPH Rattus norvegicus rate-limiting enzyme of thymidine and uridine degradation dihydrothymine + NADP+
-
 r
1.3.1.2 uracil + NADPH Rattus norvegicus
-
 5,6-dihydrouracil + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 4.7
-
 substrate nitouracil Rattus norvegicus
1.3.1.2 20.9
-
 substrate 5-iodouracil Rattus norvegicus
1.3.1.2 21.5
-
 substrate 5-diazouracil Rattus norvegicus
1.3.1.2 27.9
-
 substrate thymine Rattus norvegicus
1.3.1.2 34.9
-
 substrate uracil Rattus norvegicus
1.3.1.2 46.9
-
 substrate 5-bromouracil Rattus norvegicus
1.3.1.2 47.4
-
 substrate 5-fluorouracil Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5,6-dihydrouracil + NADP+
-
 Rattus norvegicus uracil + NADPH
-
 r
1.3.1.2 5-bromouracil + NADPH
-
 Rattus norvegicus 5-bromo-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-diazouracil + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Rattus norvegicus 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-iodouracil + NADPH
-
 Rattus norvegicus 5-iodo-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 5-nitrouracil + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.2 dihydrothymine + NADP+
-
 Rattus norvegicus thymine + NADPH
-
 r
1.3.1.2 thymine + NADPH
-
 Rattus norvegicus dihydrothymine + NADP+
-
 r
1.3.1.2 thymine + NADPH rate-limiting enzyme of thymidine and uridine degradation Rattus norvegicus dihydrothymine + NADP+
-
 r
1.3.1.2 uracil + NADPH
-
 Rattus norvegicus 5,6-dihydrouracil + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.1.2 dimer 2 * 110000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.2 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.2 45
-
 t1/2: 2 min without 2-mercaptoethanol, 2.5 min with 5 mM 2-mercaptoethanol Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.2 7.4
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 flavin enzyme contains 4 mol of FAD per mol of enzyme Rattus norvegicus
1.3.1.2 NADP+
-
 Rattus norvegicus
1.3.1.2 NADPH
-
 Rattus norvegicus