EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.4.1 | Y177A | 11% of wild-type activity in enzyme complex | Escherichia coli |
1.2.4.1 | Y177F | 7% of wild-type activity in enzyme complex | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.1 | thiamine 2-thiothiazolone diphosphate | - |
Escherichia coli | |
1.2.4.1 | thiamine 2-thiothiazolone diphosphate | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.00158 | - |
thiamine diphosphate | wild-type enzyme | Escherichia coli | |
1.2.4.1 | 0.00665 | - |
thiamine diphosphate | Y177A mutant enzyme | Escherichia coli | |
1.2.4.1 | 0.28 | - |
pyruvate | Y177A mutant enzyme | Escherichia coli | |
1.2.4.1 | 0.515 | - |
pyruvate | wild-type enzyme | Escherichia coli | |
1.2.4.1 | 0.531 | - |
pyruvate | Y177F mutant enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Escherichia coli | - |
- |
- |
1.2.4.1 | Homo sapiens | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.4.1 | 0.0048 | - |
Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate | Escherichia coli |
1.2.4.1 | 0.00671 | - |
Y177F mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate | Escherichia coli |
1.2.4.1 | 0.0098 | - |
wild-type enzyme, 2,6-dichlorophenolindophenol assay, substrate 2alpha-hydroxyethyl-thiamin diphosphate | Escherichia coli |
1.2.4.1 | 0.087 | - |
Y177A mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate | Escherichia coli |
1.2.4.1 | 0.216 | - |
Y177F mutant enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate | Escherichia coli |
1.2.4.1 | 0.445 | - |
wild-type enzyme, 2,6-dichlorophenolindophenol assay, substrate pyruvate | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.1 | acetaldehyde + benzaldehyde | - |
Escherichia coli | (R)-phenylacetylcarbinol | - |
? |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.1 | 0.000003 | - |
thiamine 2-thiazolone diphosphate | - |
Escherichia coli | |
1.2.4.1 | 0.000064 | - |
thiamine 2-thiothiazolone diphosphate | - |
Escherichia coli | |
1.2.4.1 | 0.0000737 | - |
thiamine 2-thiothiazolone diphosphate | - |
Homo sapiens |