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Literature summary extracted from
- Molecular properties of L-kynurenine 3-hydroxylase from rat liver mitochondria (1977), J. Biochem., 81, 1413-1425.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.13.9 | solubilized enzyme is appreciably stabilized in 0.05 M Tris-acetate buffer, pH 8.1, 1 mM DTT, 0.2 M mannitol, 0.2% Triton X-100, 0.005 mM FAD | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.9 | 0.00012 | - |
FAD | - |
Rattus norvegicus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.9 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 160000 | - |
2 * 160000 in 0.2% Triton-X100, SDS-PAGE | Rattus norvegicus |
| 1.14.13.9 | 345000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.9 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.9 | homogeneity | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.9 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | additional information | - |
specific activities of apoenzyme and holoenzyme with different electron acceptors | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.9 | L-kynurenine + NADPH + O2 | - |
Rattus norvegicus | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
| 1.14.13.9 | additional information | catalyzes NADH- and NADPH-linked reductions of low molecular weight acceptors such as 2,6-dichlorophenolindophenol and ferricyanide | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.9 | dimer | 2 * 160000 in 0.2% Triton-X100, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.9 | 30 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.9 | FAD | flavoprotein | Rattus norvegicus | |
| 1.14.13.9 | FAD | 4 mol of FAD per subunit | Rattus norvegicus | |
| 1.14.13.9 | NADH | - |
Rattus norvegicus | |
| 1.14.13.9 | NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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